Parrish J J, Foote R H
J Androl. 1987 Jul-Aug;8(4):259-66. doi: 10.1002/j.1939-4640.1987.tb03319.x.
The number of bovine spermatozoa separated in a swim-up procedure was quantified using an electronic cell counter. In an initial test of the swim-up procedure, non-frozen sperm samples with different ratios of live to dead cells were prepared and tested for the number of spermatozoa counted by the swim-up procedure. In ejaculates from six bulls, the number of spermatozoa swimming up was related to the number of live cells present (R2 = 0.97). Next, sperm quality of frozen-thawed semen immediately after thawing was measured at 37 C by swim-up sperm count, sperm motility, spermatozoa with an intact acrosome and migration in polyacrylamide gel and then compared with the fertility of the semen used for artificial insemination. Twenty-nine ejaculates of frozen-thawed semen from 11 bulls were evaluated. Correlations with fertility were highest on an ejaculate basis for motility (r = 0.41, P = 0.05) and for swim-up sperm count (r = 0.35, P = 0.06). On a bull basis, swim-up sperm count had the highest correlation with fertility (r = 0.59, P = 0.06). In a multiple regression model to predict male fertility that included all described measures of semen quality, a R2 value of 0.69 was obtained. This is the first report showing that the ability of spermatozoa to swim out of a more dense medium (whole milk-glycerol extender) into culture media is quantitatively related to in vivo fertility.
使用电子细胞计数器对在游动法中分离出的牛精子数量进行定量。在游动法的初步测试中，制备了具有不同活细胞与死细胞比例的非冷冻精子样本，并对通过游动法计数的精子数量进行了测试。在来自六头公牛的射精样本中，向上游动的精子数量与存在的活细胞数量相关（R2 = 0.97）。接下来，在37℃下通过游动精子计数、精子活力、顶体完整的精子以及在聚丙烯酰胺凝胶中的迁移来测量解冻后立即解冻的冷冻精液的精子质量，然后将其与用于人工授精的精液的生育力进行比较。对来自11头公牛的29份冷冻解冻精液射精样本进行了评估。在射精样本基础上，活力（r = 0.41，P = 0.05）和游动精子计数（r = 0.35，P = 0.06）与生育力的相关性最高。在公牛基础上，游动精子计数与生育力的相关性最高（r = 0.59，P = 0.06）。在一个预测雄性生育力的多元回归模型中，该模型纳入了所有描述的精液质量指标，得到的R2值为0.69。这是第一份报告表明精子从密度更高的介质（全脂牛奶 - 甘油稀释剂）游入培养基的能力与体内生育力在数量上相关。
