关于布兰汉菌属和奈瑟菌属对三丁酸甘油酯水解作用的一则笔记。

A note on hydrolysis of tributyrin by Branhamella and Neisseria.

作者信息

Riley T V

出版信息

J Appl Bacteriol. 1987 Jun;62(6):539-42. doi: 10.1111/j.1365-2672.1987.tb02686.x.

DOI:10.1111/j.1365-2672.1987.tb02686.x

Abstract

Sixty-three strains of Branhamella and Neisseria were tested by two methods for their ability to hydrolyse glycerol tributyrate. After the conventional plate test, gas liquid chromatographical (GLC) analysis of the agar medium was carried out to detect the hydrolysis product, butyric acid, and other volatile fatty acids. All strains of Branhamella catarrhalis, Neisseria caviae, N. cuniculi and N. ovis but no other Neisseria spp. gave positive results with the conventional test. With GLC, however, most strains of Branhamella and Neisseria were shown to liberate butyric acid. In addition, some strains liberated acetic and isovaleric acids. Greater amounts of butyric acid were produced by clinical strains, in particular B. catarrhalis, compared with reference strains. It was concluded that the conventional plate test for tributyrin hydrolysis differentiates B. catarrhalis, N. caviae, N. cuniculi and N. ovis from other Neisseria.

摘要

采用两种方法对63株布兰汉菌属和奈瑟菌属细菌进行甘油三丁酸酯水解能力测试。在进行常规平板试验后，对琼脂培养基进行气液色谱（GLC）分析，以检测水解产物丁酸和其他挥发性脂肪酸。所有卡他布兰汉菌、豚鼠奈瑟菌、兔奈瑟菌和羊奈瑟菌菌株，但其他奈瑟菌属菌株均未在常规试验中得到阳性结果。然而，通过GLC分析，大多数布兰汉菌属和奈瑟菌属菌株都能释放出丁酸。此外，一些菌株还能释放出乙酸和异戊酸。与参考菌株相比，临床菌株，特别是卡他布兰汉菌产生的丁酸量更多。得出结论，常规的甘油三丁酸酯水解平板试验可将卡他布兰汉菌、豚鼠奈瑟菌、兔奈瑟菌和羊奈瑟菌与其他奈瑟菌区分开来。