同时液相色谱法测定养殖鱼类中残留的合成抗菌剂。
Simultaneous liquid chromatographic determination of residual synthetic antibacterials in cultured fish.
作者信息
Nose N, Hoshino Y, Kikuchi Y, Horie M, Saitoh K, Kawachi T, Nakazawa H
出版信息
J Assoc Off Anal Chem. 1987 Jul-Aug;70(4):714-7.
A liquid chromatographic method is described to determine simultaneously the following 11 synthetic antibacterial agents used in a fishery: nitrofuran derivatives furazolidone, nifurpirinol, difurazone, and furamizole; sulfa drugs sulfamerazine, sulfisozole, sulfamonomethoxine, and sulfadimethoxine; and, oxolinic, nalidixic, and piromidic acids. A Nucleosil C18 column was used with tetrahydrofuran-acetonitrile-phosphoric acid-water (29 + 1 + 0.06 + 69.94) as the mobile phase. Pretreatment of the fish meat sample with acetone extraction and alumina column cleanup gave good separation of the LC peaks without interference from any other components. Recovery of the antibacterial agents was ca 80%. The lower limit of detection of the drugs was 1-2 ng for 10 microL injection.
本文描述了一种液相色谱法，用于同时测定渔业中使用的以下11种合成抗菌剂：硝基呋喃衍生物呋喃唑酮、硝呋吡醇、二呋咱、呋吗唑；磺胺类药物磺胺甲基嘧啶、磺胺异噁唑、磺胺间甲氧嘧啶、磺胺二甲氧嘧啶；以及恶喹酸、萘啶酸和吡咯酸。使用Nucleosil C18柱，以四氢呋喃 - 乙腈 - 磷酸 - 水（29 + 1 + 0.06 + 69.94）作为流动相。鱼肉样品经丙酮萃取和氧化铝柱净化预处理后，液相色谱峰得到良好分离，无其他任何成分干扰。抗菌剂的回收率约为80%。进样量为10 μL时，药物的检测下限为1 - 2 ng。
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