Nagata T, Saeki M
J Assoc Off Anal Chem. 1986 May-Jun;69(3):448-50.
A liquid chromatographic (LC) method is described for determination of ampicillin residues in fish tissues. The drug is extracted from tissues with methanol, and the extract is evaporated to dryness. This residue is cleaned up by Florisil cartridge chromatography. LC analysis is carried out on a Nucleosil C18 column, and ampicillin is quantitated by ultraviolet detection at 222 nm. Recoveries of ampicillin added to tissues at levels of 0.2 and 0.1 ppm were 73.2 and 61.5%, respectively. The detection limit was 3 ng for ampicillin standard, and 0.03 ppm in tissues.
本文描述了一种用于测定鱼组织中氨苄西林残留量的液相色谱（LC）方法。该药物用甲醇从组织中提取，提取物蒸发至干。此残留物通过弗罗里硅土柱色谱法进行净化。液相色谱分析在Nucleosil C18柱上进行，氨苄西林通过在222nm处的紫外检测进行定量。添加到组织中浓度为0.2ppm和0.1ppm的氨苄西林回收率分别为73.2%和61.5%。氨苄西林标准品的检测限为3ng，组织中的检测限为0.03ppm。
