Mitochondrial and cytoplasmic protein syntheses are not required for heat shock acquisition of ethanol and thermotolerance in yeast.

Heat shock acquisition of ethanol- and thermotolerance in Saccharomyces cerevisiae was not inhibited in cells incubated in the presence of cycloheximide or chloramphenicol. Respiratory-deficient (rho-) mutants also characteristically exhibited the heat shock response. It was concluded that mitochondrial and cytoplasmic protein syntheses are not required for heat shock acquisition of ethanol and thermotolerance in yeast.