Milillo C, Falcone L, Di Carlo P, Aruffo E, Del Boccio P, Cufaro M C, Patruno A, Pesce M, Ballerini P
Department of Innovative Technologies in Medicine & Dentistry, University G. d'Annunzio, 66100 Chieti, Italy; Center for Advanced Studies and Technology (CAST), "G. d'Annunzio" University of Chieti-Pescara, Chieti, Italy.
Department of Innovative Technologies in Medicine & Dentistry, University G. d'Annunzio, 66100 Chieti, Italy.
Respir Physiol Neurobiol. 2023 Jan;307:103979. doi: 10.1016/j.resp.2022.103979. Epub 2022 Oct 13.
Ozone (O) is one of the most harmful urban pollutants, but its biological mechanisms have not been fully elucidated yet. Human bronchial epithelial cells (HBEpC) and human macrophage cells (differentiated human monocytic cell line) were exposed to O at the concentration of 240 μg/m (120 ppb), corresponding to the European Union alert threshold. Cell viability, reactive oxygen species (ROS) production, and pro-inflammatory cytokines release (IL-8 and TNF-α) were evaluated. Results indicated that O exposure increases ROS production in both cell types and enhances cytokines release in macrophages. O stimulated IL-8 and TNF-α in HBEpC when the cells were pretreated with Lipopolysaccharide, used to mimic a pre-existing inflammatory condition. Proteomics analysis revealed that, in HBEpC, O caused the up-regulation of aldo-keto reductase family 1 member B10, a recognized critical protein in lung carcinogenesis. In conclusion, our results show that 120 ppb O can lead to potential damage to human health suggesting the need for a revision of the actual alert levels.
臭氧（O₃）是最有害的城市污染物之一，但其生物学机制尚未完全阐明。将人支气管上皮细胞（HBEpC）和人巨噬细胞（分化的人单核细胞系）暴露于浓度为240 μg/m³（120 ppb）的臭氧中，该浓度对应于欧盟警报阈值。评估了细胞活力、活性氧（ROS）生成以及促炎细胞因子释放（IL-8和TNF-α）。结果表明，暴露于臭氧会增加两种细胞类型中的ROS生成，并增强巨噬细胞中的细胞因子释放。当用脂多糖预处理细胞以模拟预先存在的炎症状态时，臭氧刺激了HBEpC中的IL-8和TNF-α。蛋白质组学分析显示，在HBEpC中，臭氧导致醛糖酮还原酶家族1成员B10上调，这是一种在肺癌发生中公认的关键蛋白。总之，我们的结果表明，120 ppb的臭氧会对人类健康造成潜在损害，这表明需要修订当前的警报水平。
