间接免疫荧光法检测抗巴尔通体 IgA 类抗体在猫抓病早期血清学诊断中的临床应用。

Clinical utility of indirect fluorescent assay for IgA class antibodies against Bartonella henselae in serodiagnosis of cat scratch disease in its early stage.

机构信息

Department of Clinical Laboratory Science, Faculty of Health Science, Yamaguchi University Graduate School of Medicine, Ube, Japan.

出版信息

Diagn Microbiol Infect Dis. 2022 Dec;104(4):115809. doi: 10.1016/j.diagmicrobio.2022.115809. Epub 2022 Sep 6.

DOI:10.1016/j.diagmicrobio.2022.115809

PMID:36244126

Abstract

The utility of IgA class antibodies for the serodiagnosis of cat scratch disease (CSD) was evaluated by developing an indirect immunofluorescent assay (IFA) using an antigen obtained by co-cultivating Bartonella henselae ATCC 49882 with Vero cells. Served for evaluation were 101 sera from patients serologically confirmed as CSD with IgG-IFA ≥1:256, and 144 sera from patients clinically suspected of CSD but not serologically confirmed. The sensitivity of the newly developed IgA-IFA in detecting the confirmed cases was 57.4% (58/101), and 75.0% in combination with IgM-IFA. As for the non-confirmed cases, IgA-IFA turned 8.3% cases (12/144) positive, 10 of whom were subsequently diagnosed as CSD of early stage from clinical courses and/or by repeated testing. The 12-case gain was regarded as a significant improvement. Hence, the diagnostic rate of early-stage CSD is expected to be increased by routinely performing IgA-IFA in addition to conventional IgG/IgM-IFA.

摘要

本研究通过用贝氏柯克斯体 ATCC49882 与非洲绿猴肾细胞(Vero)共同培养获取抗原，建立了间接免疫荧光法(IFA)，评估了 IgA 类抗体在猫抓病(CSD)血清学诊断中的应用。用于评估的血清样本包括 101 例经 IgG-IFA 检测 IgG 抗体≥1:256 且临床确诊的 CSD 患者血清，以及 144 例临床疑似但未经血清学确诊的 CSD 患者血清。新建立的 IgA-IFA 检测确诊病例的敏感性为 57.4%(58/101)，与 IgM-IFA 联合检测的敏感性为 75.0%。对于未确诊的病例，IgA-IFA 检测出 8.3%(12/144)的阳性病例，其中 10 例从临床过程和/或重复检测中被诊断为早期 CSD。这 12 例新增的确诊病例被认为是显著的改善。因此，通过常规进行 IgA-IFA 检测，有望提高早期 CSD 的诊断率，这一方法可作为 IgG/IgM-IFA 检测的补充。