Department of Clinical Laboratory Science, Faculty of Health Sciences, Yamaguchi University Graduate School of Medicine, Ube, Japan.
Department of Clinical Laboratory Science, Faculty of Health Sciences, Yamaguchi University Graduate School of Medicine, Ube, Japan
J Clin Microbiol. 2017 Dec 26;56(1). doi: 10.1128/JCM.01322-17. Print 2018 Jan.
We evaluated the utility of Western blot (WB) bands of e in detecting anti- immunoglobulin M (IgM) for serodiagnosis of cat scratch disease (CSD). IgM band patterns were examined using sera from 92 patients clinically suspected of having CSD and from 130 healthy individuals. Positive WB bands were observed in 49 (53.5%) of the 92 patient sera. Three bands at 8 to 10, 31 to 35, and 70 kDa were regarded as relevant for because all of the positive sera yielded at least one of the three bands, and none of the healthy control sera showed reactivity to any of them. In contrast, the positive rate of the patient sera by conventional indirect fluorescence antibody assay (IFA) for IgM was 28.3% (26/92) among the patients. These finding suggest that the IgM-WB assay, although cumbersome to perform, can be used for confirmatory diagnosis of CSD with no false positivity in the control sera. Purification of proteins in the specific bands may contribute to the development of an IgM enzyme-linked immunosorbent assay (IgM-ELISA) with improved specificity and sensitivity.
我们评估了 Western blot（WB）条带 e 在检测抗免疫球蛋白 M（IgM）中的效用，用于猫抓病（CSD）的血清学诊断。使用 92 例临床疑似患有 CSD 的患者血清和 130 例健康个体血清检查 IgM 条带模式。在 92 例患者血清中观察到 49 例（53.5%）阳性 WB 带。8 至 10、31 至 35 和 70 kDa 的三个条带被认为与 有关，因为所有阳性血清至少产生了其中一个条带，而没有任何健康对照血清对它们中的任何一个产生反应。相比之下，在这些患者中，传统间接荧光抗体测定（IFA）检测 IgM 的患者血清阳性率为 28.3%（26/92）。这些发现表明，尽管 IgM-WB 检测繁琐，但在对照血清中没有假阳性，可以用于 CSD 的确认诊断。特定条带中蛋白质的纯化可能有助于开发具有改善特异性和敏感性的 IgM 酶联免疫吸附测定（IgM-ELISA）。
