在通过仓鼠试验评估人类精子受精能力时使用短和长精子预孵育期的重要性。

Importance of the use of short and long sperm pre-incubation periods in assessing the fertilizing capacity of human spermatozoa by hamster test.

作者信息

Wramsby H, Liedholm P

出版信息

Hum Reprod. 1986 Apr;1(3):171-3. doi: 10.1093/oxfordjournals.humrep.a136374.

DOI:10.1093/oxfordjournals.humrep.a136374

Abstract

The fertilizing capacity of semen samples of seven fertile men was studied in vitro using zona-free hamster ova (hamster test). Spermatozoa of each sample were pre-incubated 4 or 24 h before ova were inseminated for 3 h. An extended insemination for an additional 3 h was also performed after the shorter pre-incubation. The overall ovum penetration rate changed from 0 to 100% (mean 52%) after short (4 h) sperm pre-incubation and from 10 to 100% (mean 55%) after long (24 h) pre-incubation. Taking into account the combination of short and long pre-incubation periods, all donors reached a penetration rate of 67 to 100%. The results indicate that both short and long pre-incubation periods must be employed when the hamster test is used diagnostically to assess the fertilizing capacity of human spermatozoa.

摘要

利用去透明带仓鼠卵（仓鼠试验）在体外研究了7名生育能力正常男性精液样本的受精能力。在卵子受精3小时前，每个样本的精子先预孵育4小时或24小时。在较短时间预孵育后，还额外延长3小时进行受精。精子短时间（4小时）预孵育后，总体卵子穿透率从0%变为100%（平均52%）；长时间（24小时）预孵育后，总体卵子穿透率从10%变为100%（平均55%）。综合考虑短时间和长时间预孵育的情况，所有供体的穿透率达到了67%至100%。结果表明，当使用仓鼠试验诊断评估人类精子的受精能力时，必须采用短时间和长时间预孵育两种方式。