Chan S Y, Tang L C
Contraception. 1984 Oct;30(4):363-9. doi: 10.1016/s0010-7824(84)80028-1.
The effect of reserpine at various concentrations (2 X 10(-6), 2 X 10(-7), 2 X 10(-8), 2 X 10(-9) and 2 X 10(-10) M) on the in vitro fertilizing capacity of human spermatozoa was studied. Spermatozoa collected from presumably fertile men were washed in BWW medium and incubated with different concentrations of reserpine for 5 hr before insemination of the zona-free hamster ova. The spermatozoal penetration of the zona-free hamster ova was scored 6 hr later and the results were analyzed statistically. Reserpine, at all the concentrations tested, caused a significant dose-dependent decrease in the penetration of the denuded hamster ova in comparison to the control (p less than 0.05). The percent motility of spermatozoa decreased as a function of time during the preincubation period to initiate spermatozoal capacitation but there were no significant differences in the values between the control and the reserpine - treated spermatozoa (p greater than 0.05). These findings indicate that reserpine can affect the fertilizing capacity of human spermatozoa in vitro and provide an additional evidence to suggest the prospective use of reserpine as a vaginal contraceptive.
研究了不同浓度（2×10⁻⁶、2×10⁻⁷、2×10⁻⁸、2×10⁻⁹和2×10⁻¹⁰ M）的利血平对人精子体外受精能力的影响。从可能具有生育能力的男性收集的精子在BWW培养基中洗涤，并在将无透明带仓鼠卵受精前，与不同浓度的利血平孵育5小时。6小时后对无透明带仓鼠卵的精子穿透情况进行评分，并对结果进行统计学分析。与对照组相比，所有测试浓度的利血平均导致去透明带仓鼠卵的精子穿透率显著呈剂量依赖性下降（p<0.05）。在预孵育期开始精子获能时，精子的活力百分比随时间下降，但对照组和利血平处理组精子之间的值无显著差异（p>0.05）。这些发现表明，利血平可在体外影响人精子的受精能力，并为利血平作为阴道避孕药的潜在用途提供了额外证据。
