Hill G B, Ayers O M, Kohan A P
J Clin Microbiol. 1987 Aug;25(8):1540-5. doi: 10.1128/jcm.25.8.1540-1545.1987.
Three new species, Eubacterium nodatum, Eubacterium timidum, and Eubacterium brachy, were described, primarily from subgingival samples taken from patients with moderate and severe adult periodontitis. Except for the isolation of E. brachy from a pleuropulmonary infection, these species have not been reported from other infected body sites. We report on the isolation of these species and an undescribed group (D-6) of asaccharolytic eubacteria also found in periodontal disease from numerous different sites of infection, mostly the head and neck. A similarity in cellular morphological properties of E. nodatum and Actinomyces sp. was noted previously. Additional similarities, particularly to Actinomyces israelii, that we found are the formation of molar tooth colonies and the isolation from cases of lumpy jaw and from the genital tract of women in association with the use of an intrauterine contraceptive device. E. timidum and E. brachy did not occur more often from any particular site outside of the head, neck, and respiratory tract. The group D-6 strains came from a variety of sites in the trunk and pelvis. These species are all obligately anaerobic, asaccharolytic, and generally nonreactive, and they grow poorly and slowly on media commonly used to isolate anaerobic bacteria. L-Lysine (0.5%) markedly stimulated the growth of E. nodatum and, to a lesser extent, another acetate- and butyrate-producing group, Eubacterium sp. group D-6, but we did not find comparable stimulants for the other species. We found the production of phenyl acetate to be a helpful marker in the identification of E. timidum and Eubacterium sp. group D-6. Although the isolation and identification of most of these species remain somewhat difficult, the evidence from dental infections and the present report suggests that these species are potential pathogens that are likely to be overlooked in infected clinical material without special attention to more prolonged incubation and use of enriched isolation media.
描述了三个新物种，即结节真杆菌、胆小真杆菌和短真杆菌，主要来自中度和重度成人牙周炎患者的龈下样本。除了从胸膜肺部感染中分离出短真杆菌外，尚未有关于这些物种在其他感染身体部位的报道。我们报告了从众多不同感染部位（主要是头颈部）的牙周病中分离出这些物种以及一组未描述的解糖真细菌（D-6）。先前已注意到结节真杆菌与放线菌属在细胞形态特性上的相似性。我们发现的其他相似之处，特别是与以色列放线菌的相似之处，包括形成磨牙样菌落以及从颌骨肿大病例和使用宫内节育器的女性生殖道中分离得到。胆小真杆菌和短真杆菌在头、颈和呼吸道以外的任何特定部位出现的频率并不更高。D-6组菌株来自躯干和骨盆的多个部位。这些物种均为专性厌氧菌，不分解糖类，通常无反应性，并且在常用于分离厌氧菌的培养基上生长不良且缓慢。L-赖氨酸（0.5%）显著刺激了结节真杆菌的生长，在较小程度上也刺激了另一个产乙酸和丁酸的菌群——解糖真杆菌D-6组的生长，但我们未找到其他物种的类似刺激物。我们发现苯乙酸的产生是鉴定胆小真杆菌和解糖真杆菌D-6组的一个有用标记。尽管分离和鉴定这些物种中的大多数仍然有些困难，但来自牙齿感染的证据和本报告表明，这些物种是潜在病原体，如果在感染的临床材料中不特别注意延长培养时间和使用富集分离培养基，很可能会被忽视。
