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应用于厌氧菌鉴定的气液色谱改良提取程序。

Modified extraction procedure for gas-liquid chromatography applied to the identification of anaerobic bacteria.

作者信息

Thomann W R, Hill G B

出版信息

J Clin Microbiol. 1986 Feb;23(2):392-4. doi: 10.1128/jcm.23.2.392-394.1986.

Abstract

Chloroform and ether commonly are used as solvents to extract metabolic organic acids for analysis by gas-liquid chromatography in the identification of anaerobic bacteria. Because these solvents are potentially hazardous to personnel, modified extraction procedures involving the use of a safer solvent, methyl tert-butyl ether were developed which remained both simple to perform and effective for organism identification.

摘要

氯仿和乙醚通常用作溶剂,用于提取代谢有机酸,以便通过气液色谱法分析来鉴定厌氧菌。由于这些溶剂对人员有潜在危害,因此开发了涉及使用更安全溶剂甲基叔丁基醚的改良提取程序,该程序操作简单且对微生物鉴定有效。

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