Thomann W R, Hill G B
J Clin Microbiol. 1986 Feb;23(2):392-4. doi: 10.1128/jcm.23.2.392-394.1986.
Chloroform and ether commonly are used as solvents to extract metabolic organic acids for analysis by gas-liquid chromatography in the identification of anaerobic bacteria. Because these solvents are potentially hazardous to personnel, modified extraction procedures involving the use of a safer solvent, methyl tert-butyl ether were developed which remained both simple to perform and effective for organism identification.
氯仿和乙醚通常用作溶剂，用于提取代谢有机酸，以便通过气液色谱法分析来鉴定厌氧菌。由于这些溶剂对人员有潜在危害，因此开发了涉及使用更安全溶剂甲基叔丁基醚的改良提取程序，该程序操作简单且对微生物鉴定有效。
