Engineering and Technology Research Center of Shellfish Breeding in Liaoning Province, College of Fisheries and Life Science, Dalian Ocean University, Dalian 116023, China.
Engineering and Technology Research Center of Shellfish Breeding in Liaoning Province, College of Fisheries and Life Science, Dalian Ocean University, Dalian 116023, China.
Sci Total Environ. 2023 Jan 20;857(Pt 2):159430. doi: 10.1016/j.scitotenv.2022.159430. Epub 2022 Oct 13.
The temperature has always been a key environmental factor in Manila clam (Ruditapes philippinarum) culture. In this study, the Manila clam was treated to different temperature pre-heat (28 °C, 30 °C) and gained heat tolerance after recover of 12 h, and a survival rate (14.7 %-49.1 %) advantage after high temperature challenge (30 and 32 °C). To further investigate the physiological and metabolism changes in Manila clam that had experienced a heat stress, non-targeted metabolomics (LC-MS/MS) was used to analyze the metabolic responses of gills in three group Manila clams during the heat challenge. Metabolic profiles revealed that high temperature caused changes in fatty acid composition, energy metabolism, antioxidant metabolites, hydroxyl compounds, and amino acids in heat-hardened clams compared to non-hardened clams. We found a number of significantly enriched pathways, including cAMP signaling pathway, serotonergic synapse, and biosynthesis of unsaturated fatty acids in heat-hardened Manila clam compared with non-hardened and untreated Manila clam. After a brief high temperature treatment, the physiological maintenance ability of Manila clam was improved. Combined with metabolomics analysis, heat hardening treatment may improve the energy metabolism and antioxidant ability of Manila clam. These results provide new insights into the cellular and metabolic responses of Manila clams following high temperature stress.
温度一直是马尼拉蛤（Ruditapes philippinarum）养殖的关键环境因素。在这项研究中，马尼拉蛤在不同的温度预热（28°C、30°C）下处理，并在 12 小时恢复后获得耐热性，在高温挑战（30 和 32°C）后具有更高的存活率（14.7%-49.1%）优势。为了进一步研究经历热应激的马尼拉蛤的生理和代谢变化，使用非靶向代谢组学（LC-MS/MS）分析了在热应激期间三组马尼拉蛤鳃中的代谢反应。代谢图谱显示，与未硬化的蛤相比，高温导致硬化蛤的脂肪酸组成、能量代谢、抗氧化代谢物、羟基化合物和氨基酸发生变化。与未硬化和未处理的马尼拉蛤相比，我们发现硬化的马尼拉蛤中有许多显著富集的途径，包括 cAMP 信号通路、血清素突触和不饱和脂肪酸的生物合成。经过短暂的高温处理，马尼拉蛤的生理维持能力得到提高。结合代谢组学分析，热硬化处理可能提高了马尼拉蛤的能量代谢和抗氧化能力。这些结果为马尼拉蛤在高温胁迫下的细胞和代谢反应提供了新的见解。
