Engineering and Technology Research Center of Shellfish Breeding in Liaoning Province, College of Fisheries and Life Science, Dalian Ocean University, Dalian, China.
College of Marine Science and Environment, Dalian Ocean University, Dalian, China.
Proteomics. 2023 May;23(9):e2100396. doi: 10.1002/pmic.202100396. Epub 2023 Mar 10.
Water temperature is one of the key environmental factors for marine ectotherms and a change in temperature beyond and organism's capacity limits can cause a series of changes to physiological state and damage to the organism. Understanding how organisms adapt to complex environments is a central goal of evolutionary biology and ecology. Ruditapes philippinarum is an ecologically and scientifically important marine bivalve species. To uncover the molecular mechanisms of acclimation of R. philippinarum to low-temperature stress, iTRAQ-based quantitative proteomics was conducted to compare the proteomes of the north and south populations of R. philippinarum under low-temperature stress. The results showed a total of 6355 and 6352 proteins were identified in two populations, respectively. Among these, 94 and 83 were differentially abundant proteins (DAPs), and most of DAPs were related to oxidation-process, protein binding, or an integral component of membrane. According to the results of KEGG pathway enrichment analysis, most of DAPs in both populations are involved in immune-related pathways, while other population-specific significant abundance proteins of south population and north population were enriched in biosynthesis of amino acids (Enolase, Glutamine synthetase) and unsaturated fatty acids pathways (3-ketoacyl-CoA thiolase, Stearoyl-CoA desaturase), respectively, indicating that two population of clams may have different cold-stress regulation mechanisms. Our study provides new insights into different cold stress tolerance mechanisms in northern and southern populations of R. philippinarum using iTRAQ-based proteomics. This work contributes to a better understanding of molecular basis on cold stress response and adaptations, which shed lights on evolutionary biology and general ecophysiology of R. philippinarum.
水温是海洋变温动物的关键环境因素之一，超过生物体承受能力的温度变化会导致一系列生理状态的变化，并对生物体造成损害。了解生物体如何适应复杂的环境是进化生物学和生态学的核心目标。菲律宾蛤仔是一种具有重要生态和科学意义的海洋双壳类动物。为了揭示菲律宾蛤仔对低温胁迫适应的分子机制，采用 iTRAQ 定量蛋白质组学技术比较了南北种群菲律宾蛤仔在低温胁迫下的蛋白质组。结果表明，两个种群分别鉴定到 6355 种和 6352 种蛋白质。其中，94 种和 83 种蛋白质为差异丰度蛋白（DAP），大多数 DAP 与氧化过程、蛋白质结合或膜的组成部分有关。根据 KEGG 途径富集分析的结果，两个种群的大多数 DAP 都参与了免疫相关途径，而其他种群特有的南种群和北种群的显著丰度蛋白则分别富集在氨基酸生物合成途径（烯醇酶、谷氨酰胺合成酶）和不饱和脂肪酸途径（3-酮酰基辅酶 A 硫解酶、硬脂酰辅酶 A 去饱和酶）中，表明两种种群的蛤仔可能具有不同的冷应激调节机制。本研究采用 iTRAQ 蛋白质组学为揭示菲律宾蛤仔南北种群的不同冷胁迫耐受机制提供了新的见解。这项工作有助于更好地理解菲律宾蛤仔对冷应激的分子基础和适应机制，为其进化生物学和一般生态生理学提供了新的认识。
