Zhang Weiping, Rau Sibylle, Kotzagiorgis Konstantinos, Rothweiler René, Nahles Susanne, Gottwald Eric, Rolauffs Bernd, Steinberg Thorsten, Nelson Katja, Altmann Brigitte
G.E.R.N Research Center for Tissue Replacement, Regeneration & Neogenesis, Department of Oral- and Craniomaxillofacial Surgery, Medical Center, University of Freiburg, Faculty of Medicine, University of Freiburg, Freiburg, Germany.
Department of Oral and Craniomaxillofacial Surgery, Medical Center, University of Freiburg, Faculty of Medicine, University of Freiburg, Freiburg, Germany.
Front Bioeng Biotechnol. 2022 Sep 28;10:918866. doi: 10.3389/fbioe.2022.918866. eCollection 2022.
In oral and maxillofacial bone reconstruction, autografts from the iliac crest represent the gold standard due to their superior clinical performance, compared to autografts derived from other extraoral regions. Thus, the aim of our study was to identify putative differences between osteoblasts derived from alveolar (hOB-A) and iliac crest (hOB-IC) bone of the same donor (nine donors) by means of their molecular properties in 2D and 3D culture. We thereby focused on the gene expression of biomarkers involved in osteogenic differentiation, matrix formation and osteoclast modulation. Furthermore, we examined the transcriptional response to Vit.D3 in hOB-A and hOB-IC. Our results revealed different modulation modes of the biomarker expression in osteoblasts, namely cell origin/bone entity-dependent, and culture configuration- and/or time-dependent modulations. SEMA3A, SPP1, BGLAP and PHEX demonstrated the strongest dependence on cell origin. With respect to Vit.D3-effects, BGLAP, SPP1 and ALPL displayed the highest Vit.D3-responsiveness. In this context we demonstrated that the transcriptional Vit.D3-response concerning SPP1 and ALPL in human osteoblasts depended on the cell origin. The results indicate a higher bone remodeling activity of iliac crest than alveolar osteoblasts and support the growing evidence that a high osteoclast activity at the host-/donor bone interface may support graft integration.
在口腔颌面骨重建中，与取自其他口腔外区域的自体骨相比，取自髂嵴的自体骨因其卓越的临床性能而成为金标准。因此，我们研究的目的是通过在二维和三维培养中研究来自同一供体（九名供体）的牙槽骨（hOB-A）和成骨细胞的分子特性，来确定它们之间的潜在差异。我们重点研究了参与成骨分化、基质形成和破骨细胞调节的生物标志物的基因表达。此外，我们检测了hOB-A和hOB-IC对维生素D3的转录反应。我们的结果揭示了成骨细胞中生物标志物表达的不同调节模式，即细胞起源/骨实体依赖性、培养配置和/或时间依赖性调节。SEMA3A、SPP1、BGLAP和PHEX对细胞起源的依赖性最强。关于维生素D3的作用，BGLAP、SPP1和ALPL表现出最高的维生素D3反应性。在此背景下，我们证明了人成骨细胞中关于SPP1和ALPL的转录维生素D3反应取决于细胞起源。结果表明，髂嵴成骨细胞的骨重塑活性高于牙槽成骨细胞，并支持越来越多的证据表明，宿主/供体骨界面处的高破骨细胞活性可能有助于移植物整合。
