Abdel Wahab Walaa A, Esawy Mona A
Chemistry of Natural and Microbial Products Dept., National Research Centre, Dokki, Giza, Egypt.
Heliyon. 2022 Oct 3;8(10):e10869. doi: 10.1016/j.heliyon.2022.e10869. eCollection 2022 Oct.
Enzyme synthesis from local wastes has a lot of potential because it eliminates the problem of waste accumulating in conjugation while also cutting the cost of these useful products. Plackett-Burman (PBD) and central composite designs (CCD) were used to optimize the manufacturing process utilizing the honey isolate EM77, resulting in a 60-fold increase in enzyme productivity using a group of wastes comprising wheat, rice straw, and sawdust. The enzyme had its optimum activity at 60 °C, pH 5, and had high thermo-stability at 60 °C, with K and V of 0.8 mg mL and 2083.33 μmol mL min respectively. The activation (E) and deactivation (E) energies of chitinase were 2.78 and 174.46 kJ mol, respectively, with the thermodynamic constants Δ° and ΔG° ensuring enzyme stability. As a result, EM77 chitinase has the efficiency to meet the global market demand for chitinase enzyme while also providing a significantly lower price than what is now available on the websites of specialist international companies. The production process is almost costless because it is based primarily on waste and contains traces of minerals.
利用当地废弃物合成酶具有很大潜力，因为它消除了废弃物堆积的问题，同时还降低了这些有用产品的成本。采用Plackett-Burman（PBD）和中心复合设计（CCD），利用蜂蜜分离物EM77优化制造工艺，使用包括小麦、稻草和锯末在内的一组废弃物，使酶产量提高了60倍。该酶在60°C、pH值为5时具有最佳活性，在60°C时具有较高的热稳定性，K和V分别为0.8 mg/mL和2083.33 μmol/mL min。几丁质酶的活化能（E）和失活能（E）分别为2.78和174.46 kJ/mol，热力学常数Δ°和ΔG°确保了酶的稳定性。因此，EM77几丁质酶有能力满足全球市场对几丁质酶的需求，同时其价格也比国际专业公司网站上目前的价格低得多。生产过程几乎没有成本，因为它主要基于废弃物，且含有微量矿物质。
