Statistical, physicochemical, and thermodynamic profiles of chitinase production from local agro-industrial wastes employing the honey isolate EM77.

Enzyme synthesis from local wastes has a lot of potential because it eliminates the problem of waste accumulating in conjugation while also cutting the cost of these useful products. Plackett-Burman (PBD) and central composite designs (CCD) were used to optimize the manufacturing process utilizing the honey isolate EM77, resulting in a 60-fold increase in enzyme productivity using a group of wastes comprising wheat, rice straw, and sawdust. The enzyme had its optimum activity at 60 °C, pH 5, and had high thermo-stability at 60 °C, with K and V of 0.8 mg mL and 2083.33 μmol mL min respectively. The activation (E) and deactivation (E) energies of chitinase were 2.78 and 174.46 kJ mol, respectively, with the thermodynamic constants Δ° and ΔG° ensuring enzyme stability. As a result, EM77 chitinase has the efficiency to meet the global market demand for chitinase enzyme while also providing a significantly lower price than what is now available on the websites of specialist international companies. The production process is almost costless because it is based primarily on waste and contains traces of minerals.