骨髓间充质干细胞来源的外泌体miR-29a促进血管生成和成骨作用。
BMSC-Derived Exosomal miR-29a Promotes Angiogenesis and Osteogenesis.
作者信息
Lu Guo-Dong, Cheng Peng, Liu Ting, Wang Zhong
机构信息
Department of Cardiology, The First Affiliated Hospital of Shihezi University Medical College, Shihezi, China.
Division of Geriatric Endocrinology, The First Affiliated Hospital, Nanjing Medical University, Nanjing, China.
出版信息
Front Cell Dev Biol. 2020 Dec 9;8:608521. doi: 10.3389/fcell.2020.608521. eCollection 2020.
Angiogenesis and osteogenesis are tightly coupled during bone modeling and remodeling processes. Here we reported that bone marrow mesenchymal stem cell (BMSC)-derived exosomal miR-29a promotes angiogenesis and osteogenesis and . BMSC-derived exosomes (BMSCs-Exos) can be taken up by human umbilical vein endothelial cells (HUVECs) and promote the proliferation, migration, and tube formation of HUVECs. MiRNA-29a level was high in BMSCs-Exos and can be transported into HUVECs to regulate angiogenesis. VASH1 was identified as a direct target of miR-29a, mediating the effects of BMSC-derived exosomal miR-29a on angiogenesis. More interestingly, miR29a-loaded exosomes from engineered BMSCs (miR-29a-loaded BMSCs-Exos) showed a robust ability of promoting angiogenesis and osteogenesis . Taken together, these findings suggest that BMSC-derived exosomal miR-29a regulates angiogenesis and osteogenesis, and miR-29a-loaded BMSCs-Exos may serve as a potential therapeutic target for osteoporosis.
在骨塑形和重塑过程中，血管生成与骨生成紧密相关。在此我们报道，骨髓间充质干细胞（BMSC）来源的外泌体miR-29a促进血管生成和骨生成。BMSC来源的外泌体（BMSCs-Exos）可被人脐静脉内皮细胞（HUVECs）摄取，并促进HUVECs的增殖、迁移和管腔形成。miRNA-29a在BMSCs-Exos中水平较高，可转运至HUVECs中以调节血管生成。VASH1被鉴定为miR-29a的直接靶标，介导BMSC来源的外泌体miR-29a对血管生成的影响。更有趣的是，来自工程化BMSC的载有miR29a的外泌体（载有miR-29a的BMSCs-Exos）显示出强大的促进血管生成和骨生成的能力。综上所述，这些发现表明BMSC来源的外泌体miR-29a调节血管生成和骨生成，且载有miR-29a的BMSCs-Exos可能作为骨质疏松症的潜在治疗靶点。
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