Li Wenjuan, Wang Limin, Qi Yanni, Xie Yaping, Zhao Wei, Dang Zhao, Zhang Jianping
Institute of Crop Research, Gansu Academy of Agricultural Sciences, Lanzhou, China.
Front Plant Sci. 2022 Sep 30;13:1003758. doi: 10.3389/fpls.2022.1003758. eCollection 2022.
Seeds of flax ( L.) are highly rich in both oil and linolenic acid (LIN). It is crucial for flax agricultural production to identify positive regulators of fatty acid biosynthesis. In this study, we find that WRINKLED1 transcription factors play important positive roles during flax seed oil accumulation. Two WRINKLED1 genes, and , were cloned from flax, and was found be expressed predominantly in developing seeds during maturation. Overexpression of increased seed size, weight, and oil content in Arabidopsis and increased seed storage oil content in transgenic flax without affecting seed production or seed oil quality. The rise in oil content in transgenic flax seeds was primarily attributable to the increase in seed weight, according to a correlational analysis. Furthermore, overexpression or interference of upregulated the expression of genes in the fatty acid biosynthesis pathway and LAFL genes, and the expression level of WRI1 was highly significantly positively associated between L1L, LEC1, and BCCP2. Our findings give a theoretical scientific foundation for the future application of genetic engineering to enhance the oil content of plant seeds.
亚麻（L.）种子富含油脂和亚麻酸（LIN）。鉴定脂肪酸生物合成的正向调节因子对亚麻农业生产至关重要。在本研究中，我们发现WRINKLED1转录因子在亚麻种子油脂积累过程中发挥重要的正向作用。从亚麻中克隆了两个WRINKLED1基因，即 和 ，并发现 在种子成熟过程中主要在发育中的种子中表达。在拟南芥中过表达 增加了种子大小、重量和油含量，并且在转基因亚麻中增加了种子储存油含量，而不影响种子产量或种子油质量。根据相关性分析，转基因亚麻种子油含量的增加主要归因于种子重量的增加。此外， 过表达或干扰上调了脂肪酸生物合成途径中的基因和LAFL基因的表达，并且WRI1的表达水平在L1L、LEC1和BCCP2之间高度显著正相关。我们的研究结果为未来应用基因工程提高植物种子油含量提供了理论科学依据。
