Ye Jian, Wang Chunming, Sun Yanwei, Qu Jing, Mao Huizhu, Chua Nam-Hai
Temasek Life Sciences Laboratory, National University of Singapore, Singapore, Singapore.
State Key Laboratory of Plant Genomics, Institute of Microbiology, Chinese Academy of Sciences, Beijing, China.
Front Plant Sci. 2018 Oct 22;9:1479. doi: 10.3389/fpls.2018.01479. eCollection 2018.
Vegetable oil is an important renewable resource for dietary consumption for human and livestock, and more recently for biodiesel production. Lipid traits in crops are controlled by multiple quantitative trait loci (QTLs) and each of them has a small effect on lipid traits. So far, there is limited success to increase lipid yield and improve lipid quality in plants. Here, we reported the identification of a homolog of APETALA2 (AP2) transcription factor WRINKLED1 (JcWRI1) from an oleaginous plant and characterized its function in and . Using physical mapping data, we located in a QTL region specifying high oleate and lipid content in . Overexpression of in elevated seed lipid content and increased seed mass. Lipid profile in seeds of over-expression plants showed higher oleate content which will be beneficial to improve biodiesel quality. Overexpression of activated lipid-related gene expression and JcWRI1 was shown to directly bind to the AW-box of promoters of some of these genes. In conclusion, we were able to increase seed lipid content and improve seed lipid quality in by manipulating one key transcription factor JcWRI1.
植物油是人类和牲畜饮食消费的重要可再生资源，最近也用于生物柴油生产。作物中的脂质性状由多个数量性状位点（QTL）控制，每个位点对脂质性状的影响都很小。到目前为止，在提高植物脂质产量和改善脂质质量方面取得的成功有限。在这里，我们报道了从一种含油植物中鉴定出APETALA2（AP2）转录因子WRINKLED1（JcWRI1）的同源物，并对其在[具体内容缺失]中的功能进行了表征。利用物理图谱数据，我们将[具体内容缺失]定位在一个指定[具体植物缺失]中高油酸和脂质含量的QTL区域。在[具体植物缺失]中过表达[具体内容缺失]提高了种子脂质含量并增加了种子质量。过表达植物种子中的脂质谱显示油酸含量较高，这将有利于提高生物柴油质量。过表达[具体内容缺失]激活了脂质相关基因的表达，并且JcWRI1被证明直接结合其中一些基因启动子的AW-box。总之，我们能够通过操纵一个关键转录因子JcWRI1来提高[具体植物缺失]的种子脂质含量并改善种子脂质质量。
