Zhu Yi, Elcin Evrim, Jiang Mengyuan, Li Boling, Wang Hailong, Zhang Xiaokai, Wang Zhenyu
School of Environmental and Civil Engineering, Institute of Environmental Processes and Pollution Control, Jiangnan University, Wuxi, China.
Department of Agricultural Biotechnology, Division of Enzyme and Microbial Biotechnology, Faculty of Agriculture, Aydın Adnan Menderes University, Aydın, Turkey.
Front Chem. 2022 Sep 30;10:1018124. doi: 10.3389/fchem.2022.1018124. eCollection 2022.
Water contamination has become increasingly a critical global environmental issue that threatens human and ecosystems' health. Monitoring and risk assessment of toxic pollutants in water bodies is essential to identifying water pollution treatment needs. Compared with the traditional monitoring approaches, environmental biosensing whole-cell bioreporters (WCBs) has exhibited excellent capabilities for detecting bioavailability of multiple pollutants by providing a fast, simple, versatile and economical way for environmental risk assessment. The performance of WCBs is determined by its elements of construction, such as host strain, regulatory and reporter genes, as well as experimental conditions. Previously, numerous studies have focused on the design and construction of WCB rather than improving the detection process and commercialization of this technology. For investigators working in the environmental field, WCB can be used to detect pollutants is more important than how they are constructed. This work provides a review of the development of WCBs and a brief introduction to genetic construction strategies and aims to summarize key studies on the application of WCB technology in detection of water contaminants, including organic pollutants and heavy metals. In addition, the current status of commercialization of WCBs is highlighted.
水污染日益成为一个严峻的全球环境问题，威胁着人类和生态系统的健康。对水体中的有毒污染物进行监测和风险评估对于确定水污染治理需求至关重要。与传统监测方法相比，环境生物传感全细胞生物报告器（WCBs）通过为环境风险评估提供一种快速、简单、通用且经济的方式，在检测多种污染物的生物可利用性方面展现出卓越能力。WCBs的性能取决于其构建要素，如宿主菌株、调控基因和报告基因，以及实验条件。此前，众多研究聚焦于WCB的设计与构建，而非改进该技术的检测过程和商业化。对于从事环境领域工作的研究人员而言，WCB用于检测污染物比其构建方式更为重要。本文综述了WCBs的发展情况，简要介绍了基因构建策略，旨在总结WCB技术在检测水污染物（包括有机污染物和重金属）方面的关键研究。此外，还强调了WCBs的商业化现状。
