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某些抗糖尿病草药提取物对人结肠癌Caco-2细胞系中胰岛素降解酶的影响。

Effects of some anti-diabetic herbal extracts on the insulin-degrading enzyme in human colon cancer Caco-2 cell line.

作者信息

Norouzi Mahtab, Saghi Hossein, Mohebbati Reza, Mirzavi Farshad, Afshari Amir Reza, Soukhtanloo Mohammad

机构信息

Department of Clinical Biochemistry, Faculty of Medicine, Mashhad University of Medical Sciences, Mashhad, Iran.

Student Research Committee, Faculty of Medicine, Mashhad University of Medical Sciences, Mashhad, Iran.

出版信息

Avicenna J Phytomed. 2022 Sep-Oct;12(5):548-558. doi: 10.22038/AJP.2022.19982.

DOI:10.22038/AJP.2022.19982
PMID:36249459
原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC9516401/
Abstract

OBJECTIVE

Type 2 diabetes mellitus (T2DM) is a condition characterized by insufficient insulin production or insulin resistance. The insulin-degrading enzyme (IDE) is responsible for degrading insulin and is a potential drug target for T2DM treatment. Numerous activities have been proposed for plant extracts, but research on the effects of plant extracts on IDE expression and activity is riddled with drawbacks.

MATERIALS AND METHODS

We investigated the effect of and extracts on the expression and activity of IDE in the Caco-2 cell line.

RESULTS

Findings of RT-PCR showed that IDE gene expression was reduced following treatment with and extracts. The results of IDE activity with fluorogenic peptide substrate V also indicated that and extracts reduced IDE activity in a significant and dose-dependent manner.

CONCLUSION

The hydroalcoholic extracts studied, except for , can prevent insulin degradation by reducing the expression and activity of the IDE enzyme. This new insight into the effects of herbal medicines on IDE activity can help future studies.

摘要

目的

2型糖尿病（T2DM）是一种以胰岛素分泌不足或胰岛素抵抗为特征的疾病。胰岛素降解酶（IDE）负责降解胰岛素，是T2DM治疗的一个潜在药物靶点。植物提取物具有多种活性，但关于植物提取物对IDE表达和活性影响的研究存在诸多缺陷。

材料与方法

我们研究了[提取物名称1]和[提取物名称2]提取物对Caco-2细胞系中IDE表达和活性的影响。

结果

RT-PCR结果显示，用[提取物名称1]和[提取物名称2]提取物处理后，IDE基因表达降低。用荧光肽底物V检测IDE活性的结果也表明，[提取物名称1]和[提取物名称2]提取物以显著的剂量依赖性方式降低了IDE活性。

结论

除了[提取物名称3]外，所研究的水醇提取物可通过降低IDE酶的表达和活性来防止胰岛素降解。这种对草药对IDE活性影响的新认识有助于未来的研究。

https://cdn.ncbi.nlm.nih.gov/pmc/blobs/b758/9516401/e7bef2826b5c/AJP-12-548-g003.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/b758/9516401/f14cd7a8b986/AJP-12-548-g001.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/b758/9516401/2f24dba0a524/AJP-12-548-g002.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/b758/9516401/e7bef2826b5c/AJP-12-548-g003.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/b758/9516401/f14cd7a8b986/AJP-12-548-g001.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/b758/9516401/2f24dba0a524/AJP-12-548-g002.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/b758/9516401/e7bef2826b5c/AJP-12-548-g003.jpg

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