Deciphering the pharmacological mechanisms of against rheumatoid arthritis by integrating network pharmacology and experimental validation.

is a plant that can be used for both food and medicine. Modern studies have shown that has anti-inflammatory and immunosuppressive effects on arthritis. However, the mechanism of action of on rheumatoid arthritis (RA) and its main active ingredients are still unclear. This study was aimed at devising an integrated strategy for investigating the bioactivity constituents and possible pharmacological mechanisms of against RA. The components of were analyzed using UPLC-Q-Exactive orbitrap MS techniques and applied to screen the active components of according to their oral bioavailability and drug-likeness index. Then, we speculated on the potential molecular mechanisms of against RA through a network pharmacology analysis. Finally, the potential molecular mechanisms of against RA were validated in a complete Freund's adjuvant (CFA)-induced RA rat model. We identified 48 components in and screened seven bioactive ingredients. The results of the network pharmacology prediction and the experimental verification results were analyzed by Venn analysis, and the experimental results concluded that mainly interferes with the inflammation of RA by inhibiting arachidonic acid metabolism and the MAPK signaling pathway. This study identified the ingredients of by UPLC-Q-Exactive orbitrap MS and explained the possible mechanisms of against RA by integrating network pharmacology and experimental validation.