Laboratory of Molecular Pharmacology, Department of Pharmacology, School of Pharmacy, Southwest Medical University, Luzhou, Sichuan, China; South Sichuan Institute of Translational Medicine, Luzhou, Sichuan, China.
Drug Research Center of Integrated Traditional Chinese and Western Medicine, Affiliated Traditional Chinese Medicine Hospital, Southwest Medical University, Luzhou, Sichuan, China.
Food Chem. 2020 Jul 30;319:126568. doi: 10.1016/j.foodchem.2020.126568. Epub 2020 Mar 5.
Penthorum chinense Pursh is a dietary medicinal plant widely distributed in Asia-Pacific countries. The present study aims to profile the chemical constituents of P. chinense and investigate its prebiotic role in modulating gut microbiota. Fifty polyphenolic compounds were rapidly identified using UPLC-HR-MS. Total flavonoid and phenolic contents of P. chinense were 46.6% and 61.3% (w/w), respectively. Thirteen individual polyphenols were quantified, which accounted for 33.1% (w/w). P. chinense induced structural arrangement of microbial community in mice, showing increased microbiota diversity, elevated Bacteroidetes/Firmicutes ratio and enriched gut health-promoting bacteria. After a one-week drug-free wash, most of these changes were recovered, but the abundance of some beneficial bacteria was further increased. The altered composition of gut microbiota enriched several metabolic pathways. Moreover, P. chinense increased antioxidant capacity in vivo. The results suggest that polyphenol-enriched P. chinense modulates gut microbiota and enhances antioxidant capacity in mice toward a beneficial environment for host health.
五匹叶是一种在亚太国家广泛分布的药食两用植物。本研究旨在对五匹叶的化学成分进行分析，并研究其作为益生菌对调节肠道微生物群的作用。采用 UPLC-HR-MS 技术快速鉴定了 50 种多酚化合物。五匹叶中总黄酮和总酚的含量分别为 46.6%和 61.3%（w/w）。共定量了 13 种单体多酚，占 33.1%（w/w）。五匹叶可诱导小鼠肠道微生物群落结构发生改变，增加微生物多样性，提高拟杆菌门/厚壁菌门比值，并富集肠道健康促进细菌。经过一周的无药物洗脱期后，这些变化大部分得到恢复，但一些有益细菌的丰度进一步增加。肠道微生物群的改变丰富了几种代谢途径。此外，五匹叶还能增加体内抗氧化能力。结果表明，富含多酚的五匹叶可调节肠道微生物群，增强小鼠的抗氧化能力，为宿主健康创造有益的环境。
