Biotoxicity assessment of cloned cry 11 protein gene from 9NF.

The current investigation describes the isolation and characterization of toxic . local isolates harboring 99% homology with prototoxin (AXJ97553.1 and novel OUB27301.1) which contains full length gene (1.9 kb). Initially, it was cloned in pTZ57R/T and then sub-cloned in pET30a(+) for expression. The optimized conditions for good expression were found 1 mM IPTG, 3.5-4 h incubation time, and 37 °C. Toxicological assays were determined against 3rd instar larvae of with expressed partially purified and crude recombinant protein using recombinant BL21, DE3 transformed with gene. It was found that partially purified protein is highly toxic against larvae with LC value of 42.883 ± 6 µg/ml. strains producing Cry 11 toxic protein can be used as biopesticide to control resistance in insects.