Fatima Naureen, Bibi Zuhra, Rehman Abdul, Bukhari DilAra Abbas
Department of Zoology, Government College University, Lahore, Pakistan.
Institute of Microbiology and Molecular Genetics, University of the Punjab, Quaid-e-Azam Campus 54590, Lahore, Pakistan.
Saudi J Biol Sci. 2023 Apr;30(4):103610. doi: 10.1016/j.sjbs.2023.103610. Epub 2023 Mar 1.
The current study was designed to evaluate the biotoxicity of screened echo-friendly strains from different areas of Pakistan. Out of 50 samples, 36% isolates were quarantined from soil containing cattle waste after morphological, biochemical, and molecular characterization. The toxicity bioassays with spores and protein diet proved that 11 isolates were utmost noxious to 3rd instar larvae of mosquitoes and . The entopathogenic activity of first 4 . toxins against was highly lethal as compared to the other dipteran larvae The toxicity (LC) of spore diet of strains GCU-DAB-NF4 (442.730 0.38 μg/ml), NF6 (460.845 0.29 μg/ml), NF3 (470.129 0.28 μg/ml), and NF7 (493.637 0.70 μg/ml) was quite high against as compared to the after 24 h of incubation. The highest toxicity of total cell protein was shown by GCU-DAB-NF4 (LC = 84.10 50 μg/ml), NF6 (95.122 0.40 μg/ml), NF3 (100.715 ± 06 μg/ml), and NF5 (103.40 ± 07 μg/ml) against after 24 h. So, these strains a have great potential to be used as biological control especially against as compared to the .
本研究旨在评估从巴基斯坦不同地区筛选出的对环境友好的菌株的生物毒性。在50个样本中，经过形态学、生化和分子特征鉴定后，36%的分离株是从含有牛粪的土壤中分离出来的。用孢子和蛋白质饲料进行的毒性生物测定证明，11株分离株对蚊子的三龄幼虫毒性极大。与其他双翅目幼虫相比，前4种毒素对其致病活性具有高度致死性。菌株GCU-DAB-NF4（442.730 ± 0.38 μg/ml）、NF6（460.845 ± 0.29 μg/ml）、NF3（470.129 ± 0.28 μg/ml）和NF7（493.637 ± 0.70 μg/ml）的孢子饲料在孵育24小时后对其毒性相当高。24小时后，GCU-DAB-NF4（LC = 84.10 ± 50 μg/ml）、NF6（95.122 ± 0.40 μg/ml）、NF3（100.715 ± 0.06 μg/ml）和NF5（103.40 ± 0.07 μg/ml）的总细胞蛋白对其毒性最高。因此，与其他相比，这些菌株有很大的潜力用作生物防治，尤其是针对。
