Biotoxicity comparison of to control vector borne diseases against mosquito fauna.

The current study was designed to evaluate the biotoxicity of screened echo-friendly strains from different areas of Pakistan. Out of 50 samples, 36% isolates were quarantined from soil containing cattle waste after morphological, biochemical, and molecular characterization. The toxicity bioassays with spores and protein diet proved that 11 isolates were utmost noxious to 3rd instar larvae of mosquitoes and . The entopathogenic activity of first 4 . toxins against was highly lethal as compared to the other dipteran larvae The toxicity (LC) of spore diet of strains GCU-DAB-NF4 (442.730  0.38 μg/ml), NF6 (460.845  0.29 μg/ml), NF3 (470.129  0.28 μg/ml), and NF7 (493.637  0.70 μg/ml) was quite high against as compared to the after 24 h of incubation. The highest toxicity of total cell protein was shown by GCU-DAB-NF4 (LC = 84.10  50 μg/ml), NF6 (95.122  0.40 μg/ml), NF3 (100.715 ± 06 μg/ml), and NF5 (103.40 ± 07 μg/ml) against after 24 h. So, these strains a have great potential to be used as biological control especially against as compared to the .