Tiersch Nolan J, Paulsen Jacqueline, Liu Yue, Tiersch Terrence R
Department of Mechanical and Industrial Engineering, Louisiana State University, Baton Rouge, LA 70803, USA.
Department of Biological and Agricultural Engineering, Louisiana State University and Agricultural Center, Baton Rouge, LA 70803, USA.
HardwareX. 2022 Oct 4;12:e00366. doi: 10.1016/j.ohx.2022.e00366. eCollection 2022 Oct.
The goal of this work was to develop prototypes of open-hardware vitrification devices for sperm cryopreservation that can be integrated with existing straw platforms. The open-hardware Vitrification Device for French Straws (VD-FS) is low-cost, customizable, 3-D printable, standardized, and allows long-term sample storage and identification. The feasibility was shown for vitrifying and storing samples with multiple configurations. The results can be improved by design alternation and evaluation of various vitrification solutions. This is the first complete open-hardware vitrification device that can be integrated with existing French-straw storage systems, providing a foundation for future community-level modifications and improvements.
这项工作的目标是开发用于精子冷冻保存的开放式硬件玻璃化设备原型，该设备可与现有的细管平台集成。用于法国细管的开放式硬件玻璃化设备（VD-FS）成本低、可定制、可3D打印、标准化，并且允许长期样本存储和识别。已证明采用多种配置对样本进行玻璃化和存储是可行的。通过设计变更和对各种玻璃化解决方案的评估可以改进结果。这是首个可与现有的法国细管存储系统集成的完整开放式硬件玻璃化设备，为未来社区层面的修改和改进奠定了基础。
