College of Chemistry and Chemical Engineering, Jiangsu University, Zhenjiang212013, China.
College of Food and Biological Engineering, Jiangsu University, Zhenjiang212013, China.
J Agric Food Chem. 2022 Nov 2;70(43):13959-13968. doi: 10.1021/acs.jafc.2c05667. Epub 2022 Oct 20.
The development of a suitable immobilization strategy to improve the performance of immobilized glucose isomerase for the isomerization of glucose to fructose is crucial to promoting the industrial production of high-fructose syrup. In this work, a novel recyclable upper critical solution temperature (UCST)-type mVBA--P(AAm--AN)@glucose isomerase biocatalyst (PVAA@GI) was prepared, and the immobilized glucose isomerase could capture the glucose substrate through the affinity of 4-vinylbenzeneboronic acid (4-VBA) and the glucose substrate, which led to the enhanced substrate affinity and catalytic efficiency of the PVAA@GI. The biocatalyst exhibited excellent stability in pH, thermal, storage, and recycling compared to the free enzyme. The mVBA--P(AAm--AN)@glucose isomerase biocatalyst displayed reversibly soluble-insoluble characteristics with temperature change, which was in the soluble state during the enzyme reaction process but could be recovered in an insoluble form by lowering the temperature after the reaction. The highest fructose production rate reached 62.79%, which would have potential application in the industrial production of high-fructose syrup.
开发合适的固定化策略来提高固定化葡萄糖异构酶将葡萄糖异构化为果糖的性能，对于促进高果糖浆的工业生产至关重要。在这项工作中，制备了一种新型可回收上临界溶液温度（UCST）型 mVBA--P(AAm--AN)@葡萄糖异构酶生物催化剂（PVAA@GI），固定化葡萄糖异构酶可以通过 4-乙烯基苯硼酸（4-VBA）和葡萄糖底物的亲和力捕获葡萄糖底物，从而提高了 PVAA@GI 的底物亲和力和催化效率。与游离酶相比，该生物催化剂在 pH 值、热稳定性、储存稳定性和回收稳定性方面表现出优异的性能。mVBA--P(AAm--AN)@葡萄糖异构酶生物催化剂具有温度变化下的可逆溶-不溶特性，在酶反应过程中处于溶解状态，但反应后降低温度可以回收为不溶形式。最高果糖得率达到 62.79%，在高果糖浆的工业生产中具有潜在的应用前景。
