亚硫酸氢盐后接头标记与高效单链 DNA 连接技术。
Post-bisulfite Adaptor Tagging with a Highly Efficient Single-Stranded DNA Ligation Technique.
机构信息
Department of Biochemistry, Kyushu University Graduate School of Medical Sciences, Fukuoka, Japan.
出版信息
Methods Mol Biol. 2023;2594:45-57. doi: 10.1007/978-1-0716-2815-7_4.
Post-bisulfite adaptor tagging (PBAT) is a procedure for efficiently preparing a sequencing library for whole-genome bisulfite sequencing (WGBS). The original version of the PBAT protocol was highly efficient, such that it helped realize library preparation from samples of limited amounts. However, two rounds of random priming reactions employed in the original protocol limited further improvement of the PBAT protocol in terms of read length and mapping rate. In this chapter, an improved version of the PBAT protocol called tPBAT is described.
亚硫酸氢盐接头标记（PBAT）是一种高效制备全基因组亚硫酸氢盐测序（WGBS）测序文库的方法。PBAT 协议的原始版本非常高效，有助于从有限量的样本中制备文库。然而，原始协议中使用的两轮随机引物反应限制了 PBAT 协议在读取长度和映射率方面的进一步改进。本章描述了 PBAT 协议的一个改进版本，称为 tPBAT。
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