用于无PCR全基因组亚硫酸氢盐测序的亚硫酸氢盐后衔接子标记法
Post-Bisulfite Adaptor Tagging for PCR-Free Whole-Genome Bisulfite Sequencing.
作者信息
Miura Fumihito, Ito Takashi
机构信息
Department of Biochemistry, Kyushu University Graduate School of Medical Sciences, 3-1-1 Maidashi, Higashi-ku, Fukuoka, 812-8582, Japan.
Core Research for Evolutional Science and Technology (CREST), Japan Agency for Medical Research and Development (AMED), 3-1-1 Maidashi, Higashi-ku, Fukuoka, 812-8582, Japan.
出版信息
Methods Mol Biol. 2018;1708:123-136. doi: 10.1007/978-1-4939-7481-8_7.
Post-bisulfite adaptor tagging (PBAT) is a highly efficient procedure to construct libraries for whole-genome bisulfite sequencing (WGBS). PBAT attaches adaptors to bisulfite-converted genomic DNA to circumvent bisulfite-induced degradation of library DNA inherent to conventional WGBS protocols. Consequently, it enables PCR-free WGBS from nanogram quantities of mammalian DNA, thereby serving as an invaluable tool for methylomics.
亚硫酸氢盐后衔接子标记（PBAT）是一种用于构建全基因组亚硫酸氢盐测序（WGBS）文库的高效方法。PBAT将衔接子连接到经亚硫酸氢盐转化的基因组DNA上，以避免传统WGBS方案中固有的亚硫酸氢盐诱导的文库DNA降解。因此，它能够从纳克量的哺乳动物DNA中进行无PCR的WGBS，从而成为甲基组学的一种宝贵工具。
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