Laboratório de Imunologia e Biologia Molecular, Instituto de Ciências da Saúde, Universidade Federal da Bahia, Salvador, Bahia State 40.110-100, Brazil.
Unidad de Inmunología Microbiana, Centro Nacional de Microbiología, Instituto de Salud Carlos III, 28220 Majadahonda, Madrid, Spain.
Comp Immunol Microbiol Infect Dis. 2022 Nov-Dec;90-91:101891. doi: 10.1016/j.cimid.2022.101891. Epub 2022 Oct 7.
The P22 ELISA was recently developed for the serodiagnosis of animal tuberculosis. Herein, the stability of the P22 antigen in different presentations and storage conditions, and the cross-reactivity with Corynebacterium pseudotuberculosis infection in small ruminants were evaluated. For the stability assay, serum samples from cows, sheep, goats, alpacas, badgers, and wild boar were used in the P22 ELISA. The cross-reactivity analysis used sera from sheep and goats with caseous lymphadenitis (CLA). Differences in the immune recognition of P22 were found when the antigen was stored at 40 °C, but without altering the negative or positive status of each sample. P22 ELISA presented 5.71 % cross-reactivity when CLA-positive sheep were evaluated, but no cross-reaction was observed among CLA-positive goat serum samples. This study showed that the P22 protein complex is stable under different formulations and temperatures, and that the assay presents a low cross-reactivity with CLA.
P22 ELISA 最近被开发用于动物结核病的血清学诊断。在此，评估了 P22 抗原在不同制剂和储存条件下的稳定性，以及与小反刍动物感染棒状杆菌伪结核的交叉反应性。为了进行稳定性检测，使用来自奶牛、绵羊、山羊、羊驼、獾和野猪的血清进行 P22 ELISA。使用患有干酪样淋巴结炎（CLA）的绵羊和山羊的血清进行交叉反应性分析。当抗原在 40°C 下储存时，发现 P22 的免疫识别存在差异，但不会改变每个样本的阴性或阳性状态。在评估 CLA 阳性绵羊时，P22 ELISA 表现出 5.71%的交叉反应性，但在 CLA 阳性山羊血清样本中未观察到交叉反应。本研究表明，P22 蛋白复合物在不同配方和温度下稳定，并且该检测方法与 CLA 的交叉反应性较低。
