Department of Stomatology, Huashan Hospital, Fudan University, Shanghai, China.
Department of Stomatology, Huashan Hospital, Fudan University, Shanghai, China.
Tissue Cell. 2022 Dec;79:101955. doi: 10.1016/j.tice.2022.101955. Epub 2022 Oct 13.
Sodium Hypochlorite (NaOCl) and Ethylene Diamine Tetraacetic Acid (EDTA) can change the biochemical and biophysical properties of dentin. However, the response of human dental pulp stem cells (hDPSCs) to NaOCl and EDTA-treated dentin remains unknown. This study was conducted to investigate the effect of NaOCl and EDTA on cell proliferation, osteogenic/odontogenic differentiation, and the response to mechanosensitive gene expression in hDPSCs. Dentin slices were treated with 5.25% NaOCl, 17% EDTA, and saline (0.9% NaCl) separately. The cell viability and osteogenic/odontogenic differentiation of hDPSCs were analyzed using scanning electron microscopy, cell counting assay, alkaline phosphatase (ALP) staining, and quantitative polymerase chain reaction (qPCR). Besides, the hardness was measured by a Vickers microhardness tester. The expression of mechanosensitive genes was detected by the qPCR assay. All the irrigant-treated dentin allowed cell attachment. The EDTA-treated dentin significantly boosted the ALP and osteogenic/odontogenic differentiation, followed by NaCl and NaOCl groups. Remarkably, these trends were similar to the expression of mechanosensitive genes but were different from the trends of hardness values. The effect of irrigant-treated dentin on regulating hDPSCs differentiation might correlate with mechanosensitive signals. Whereas, the hardness changes between groups might not produce significant roles in regulating osteogenic/odontogenic differentiation of stem cells on dentin surfaces.
次氯酸钠（NaOCl）和乙二胺四乙酸（EDTA）可以改变牙本质的生化和物理特性。然而，人类牙髓干细胞（hDPSCs）对 NaOCl 和 EDTA 处理牙本质的反应尚不清楚。本研究旨在探讨 NaOCl 和 EDTA 对 hDPSCs 增殖、成骨/成牙本质分化以及对机械敏感基因表达的反应的影响。分别用 5.25% NaOCl、17% EDTA 和生理盐水（0.9% NaCl）处理牙本质片。通过扫描电子显微镜、细胞计数法、碱性磷酸酶（ALP）染色和定量聚合酶链反应（qPCR）分析 hDPSCs 的细胞活力和成骨/成牙本质分化。此外，用维氏显微硬度计测量硬度。通过 qPCR 检测机械敏感基因的表达。所有冲洗剂处理的牙本质都允许细胞附着。EDTA 处理的牙本质显著促进了 ALP 和成骨/成牙本质分化，其次是 NaCl 和 NaOCl 组。值得注意的是，这些趋势与机械敏感基因的表达相似，但与硬度值的趋势不同。冲洗剂处理牙本质对调节 hDPSCs 分化的作用可能与机械敏感信号有关。然而，各组之间的硬度变化可能不会在牙本质表面调节干细胞成骨/成牙本质分化方面发挥重要作用。
