Department of Endodontics, Faculty of Dentistry, Hacettepe University, 06100, Ankara, Turkey.
Department of Histology and Embryology, Faculty of Medicine, Hacettepe University, Ankara, Turkey.
Odontology. 2021 Apr;109(2):313-320. doi: 10.1007/s10266-020-00545-5. Epub 2020 Aug 7.
To investigate the effect of benzalkonium chloride (BAC) addition to ethylenediaminetetraacetic acid (EDTA) on transforming growth factor-β1 (TGF-β1) release, as well as attachment and proliferation of dental pulp stem cells (DPSCs) on dentin. A total of standard 268 human dentin disks were prepared and immersed in 1.5% sodium hypochlorite (NaOCl) for 5 min. The disks were rinsed with distilled water and randomly divided into seven groups. In control group, the disks received no further treatment. The remaining disks were immersed in following solutions: 17% EDTA or 17% EDTA + 0.008% BAC for 1, 5 or 10 min and rinsed with distilled water. DPSCs were seeded in part of the disks since the TGF-β1 release assay was performed with disks with and without cells. The attachment and proliferation of DPSCs on dentin disks were analyzed using lactate dehydrogenase activity and WST-1 assays, respectively. The cell morphology was observed by scanning electron microscopy. The release of TGF-β1 was quantified using ELISA. Data were analyzed using three- and two-way analysis of variance with Bonferroni corrections. Both EDTA solutions increased the attachment and proliferation of DPSCs (p < .05) while there was no significant difference between them (p > .05). The exposure time of both EDTA solutions had no influence on cell attachment, proliferation and TGF-β1 release (p > .05). There was no significant difference in TGF-β1 release between the control and experimental groups (p > .05). The amount of released TGF-β1 from dentin disks was similar whether or not they were seeded with cells (p > .05). Dentin treatment with either of the EDTA solutions had no effect on the amount of TGF-β1 release while both EDTA solutions improved cell attachment and proliferation on dentin surface regardless of exposure time.
为了研究苯扎氯铵（BAC）添加到乙二胺四乙酸（EDTA）中对转化生长因子-β1（TGF-β1）释放的影响，以及牙髓干细胞（DPSCs）在牙本质上的黏附增殖作用。共制备 268 个标准的人牙本质圆盘，并用 1.5%次氯酸钠（NaOCl）浸泡 5 分钟。用蒸馏水冲洗牙本质圆盘，然后随机分为 7 组。在对照组中，牙本质圆盘不进行进一步处理。其余牙本质圆盘分别浸泡在以下溶液中：17% EDTA 或 17% EDTA+0.008% BAC 1、5 或 10 分钟，并用蒸馏水冲洗。一部分牙本质圆盘进行了牙髓干细胞（DPSCs）接种，因为 TGF-β1 释放试验是在有细胞和无细胞的牙本质圆盘上进行的。通过乳酸脱氢酶活性和 WST-1 检测分析 DPSCs 在牙本质圆盘上的黏附和增殖情况，通过扫描电子显微镜观察细胞形态。采用 ELISA 定量检测 TGF-β1 的释放。采用三因素和双因素方差分析，并用 Bonferroni 校正进行分析。两种 EDTA 溶液均能增加 DPSCs 的黏附和增殖（p<0.05），但两者之间无显著性差异（p>0.05）。两种 EDTA 溶液的暴露时间对细胞黏附、增殖和 TGF-β1 释放均无影响（p>0.05）。对照组和实验组之间 TGF-β1 的释放无显著性差异（p>0.05）。无论牙本质圆盘是否接种细胞，释放的 TGF-β1 量相似（p>0.05）。用 EDTA 溶液处理牙本质不会影响 TGF-β1 的释放量，而无论暴露时间如何，两种 EDTA 溶液均能改善牙本质表面细胞的黏附增殖作用。
