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四种益智果皮的比较代谢组学分析

Comparative metabolomics analysis of pericarp from four varieties of Maxim.

机构信息

Zanthoxylum Bungeanum Research Institute, Longnan Economic Forest Research Institute, Longnan, China.

Key Laboratory of Chemistry of Northwestern Plant Resources and Key Laboratory for Natural Medicine of Gansu Province, Lanzhou Institute of Chemical Physics, Chinese Academy of Sciences, Lanzhou, China.

出版信息

Bioengineered. 2022 Jun;13(6):14815-14826. doi: 10.1080/21655979.2022.2108632.

Abstract

A qualitative and quantitative analysis of metabolites was performed by metabolomics comparation on the pericarps of four varieties of Maxim. The as scion combined with three rootstock varieties of Maxim (YJ), July Maxim (QJ), and August Maxim (BJ), at the same time seedlings breeding were compared as control (MJ). A total of 1429 metabolites were identified in Maxim pericarps based on chromatography and mass spectrometry dual detection platform. While the metabolites between four varieties of varied, there was identified 31, 15, 7, 79, 42, 19 down-regulated and 55, 50, 13, 75, 43, 27 up-regulated differential metabolites between MJ and BJ, MJ and QJ, MJ and YJ, QJ and BJ, YJ and BJ, YJ and QJ. Meanwhile, the differential metabolites composition was distinct among various varieties of and dominant by phenolic compounds flavonoid and phenolic acids, especially highest in varieties July Maxim. Highlight A comparative metabolomics analyzed in four varieties of pericarp.Total 1429 metabolites were identified and mainly in flavonoid and phenolic acid.July and August Maxim has highest antioxidant capacity.The rootstock July Maxim was recommended in Loess Plateau.

摘要

采用代谢组学比较的方法,对四种厚皮甜瓜果皮中的代谢物进行了定性和定量分析。以三种砧木品种(七月厚皮甜瓜、七月王甜瓜、八月厚皮甜瓜)为接穗,与八月厚皮甜瓜(BJ)进行了嫁接,同时以实生苗(MJ)为对照进行了幼苗繁殖比较。基于色谱和质谱双重检测平台,在厚皮甜瓜果皮中鉴定出了 1429 种代谢物。四种厚皮甜瓜之间的代谢物存在差异,MJ 与 BJ、MJ 与 QJ、MJ 与 YJ、QJ 与 BJ、YJ 与 BJ、YJ 与 QJ 之间分别鉴定出 31、15、7、79、42、19 种下调和 55、50、13、75、43、27 种上调差异代谢物。同时,不同品种厚皮甜瓜之间的差异代谢物组成明显不同,以类黄酮和酚酸等酚类化合物为主,其中七月厚皮甜瓜含量最高。

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