Lee Ye-Won, Pyo Young-Hee
Department of Food and Nutrition, Sungshin Women's University, 76-Gagil Dobong-ro, Kangbuk-Gu, Seoul, 01133 South Korea.
Food Sci Biotechnol. 2022 Jul 28;31(12):1583-1591. doi: 10.1007/s10068-022-01146-4. eCollection 2022 Nov.
MV was reported to have beneficial effects in ameliorating insulin resistance in mice, but the intrinsic mechanisms for glucose homeostasis are unclear. This study examined the anti-diabetic mechanism of MV using HepG2 cells and C57BL/KsJ- mice. MV increased insulin sensitivity by promoting insulin-dependent glucose uptake and activating glycogen accumulation in HepG2 cells. Furthermore, the glucose homeostasis was enhanced in mice administered 1 mg/kg/day of MV for eight weeks by activating the IRS-1/PI3K/Akt and AMPK pathways in the skeletal muscle and liver tissue. In addition, MV promoted glycogen synthesis by regulating the key enzymes, including GSK-3β and GS, and suppressed gluconeogenesis by inhibiting the mRNA expressions of G6pase and PEPCK. These findings show that MV regulates both signaling pathways and improves the glucose metabolism disorder. Thus, MV might be an alternative functional food or nutraceutical in ameliorating T2DM.
The online version contains supplementary material available at 10.1007/s10068-022-01146-4.
据报道，二甲戊灵对改善小鼠胰岛素抵抗具有有益作用，但葡萄糖稳态的内在机制尚不清楚。本研究使用HepG2细胞和C57BL/KsJ-小鼠研究了二甲戊灵的抗糖尿病机制。二甲戊灵通过促进胰岛素依赖性葡萄糖摄取和激活HepG2细胞中的糖原积累来提高胰岛素敏感性。此外，通过激活骨骼肌和肝脏组织中的IRS-1/PI3K/Akt和AMPK途径，连续八周每天给小鼠施用1mg/kg的二甲戊灵可增强葡萄糖稳态。此外，二甲戊灵通过调节关键酶（包括GSK-3β和GS）来促进糖原合成，并通过抑制G6pase和PEPCK的mRNA表达来抑制糖异生。这些发现表明，二甲戊灵调节信号通路并改善葡萄糖代谢紊乱。因此，二甲戊灵可能是改善2型糖尿病的一种替代功能性食品或营养保健品。
在线版本包含可在10.1007/s10068-022-01146-4获取的补充材料。
