Department of Cardiology, The First Affiliated Hospital of Chongqing Medical University, Chongqing, 400016, China.
Department of Pulmonary and Critical Care Medicine, The First Affiliated Hospital of Chongqing Medical University, Chongqing, 400016, China.
Biochem Biophys Res Commun. 2022 Apr 16;600:109-116. doi: 10.1016/j.bbrc.2022.02.057. Epub 2022 Feb 17.
We aimed to examine the effect of Honokiol (HKL) on endothelial dysfunction in type 2 diabetic rats and its possible mechanism. A high-fat diet and streptozotocin (STZ) were used to establish the type 2 diabetic model in rats. Part of these rats were intraperitoneally injected with HKL 10 mg/kg daily. Then the expression of Ser1177 phosphorylation of endothelial nitric oxide synthase (p-eNOS), eNOS, and CD31, vasodilation function, insulin signaling, indicators of oxidative stress and relative signaling pathway were measured. Human umbilical vein endothelial cells (HUVECs) were used to explore the underlying mechanism of the effect of HKL on high glucose-related endothelial injury in vitro. The data showed that HKL could reverse the decline of the expression of p-eNOS and CD31, endothelium-related vasodilation dysfunction, insulin resistance and activation of oxidative stress induced by type 2 diabetes in vivo. The similar results were obtained in vitro. In summary, our study demonstrates that HKL improves endothelial function and diminishes insulin resistance and oxidative stress, suggesting that HKL could be used as a treatment option for diabetes in the future.
观察和厚朴酚（HKL）对 2 型糖尿病大鼠内皮功能障碍的影响及其可能机制。
采用高脂饮食联合链脲佐菌素（STZ）建立 2 型糖尿病大鼠模型，部分大鼠每日腹腔注射 HKL（10mg/kg）。然后检测内皮型一氧化氮合酶（eNOS）丝氨酸 1177 位磷酸化（p-eNOS）、eNOS 和 CD31 的表达、血管舒张功能、胰岛素信号、氧化应激指标及相关信号通路。
HKL 可逆转 2 型糖尿病大鼠 p-eNOS 和 CD31 表达下降、内皮相关血管舒张功能障碍、胰岛素抵抗及氧化应激激活。
体内、外研究结果均表明，HKL 可改善内皮功能，减轻胰岛素抵抗和氧化应激，提示 HKL 未来可作为糖尿病的一种治疗选择。
