Lecomte C M, Renard A, Martial J A
Nucleic Acids Res. 1987 Aug 25;15(16):6331-48. doi: 10.1093/nar/15.16.6331.
From a human pituitary cDNA library, we have cloned 3 distinct human growth hormone (hGH) cDNAs, coding respectively for the 22 K hGH, the 20 K variant, and a yet unknown 17.5 K variant. S1 mapping analysis using human pituitary RNA confirms the existence of at least four distinct hGH mRNAs originating from alternative acceptor sites at the second intron of the primary transcript. We have analysed the hGH gene sequence to explain the high frequency of alternative splicings which occur only at this location. In this study we propose CTTGNNPyPyPy as an additional consensus sequence guiding the selection of the branched nucleotide.
从人垂体cDNA文库中，我们克隆了3种不同的人生长激素（hGH）cDNA，它们分别编码22K的hGH、20K变体以及一种未知的17.5K变体。利用人垂体RNA进行的S1图谱分析证实，至少有四种不同的hGH mRNA源自初级转录本第二个内含子处的可变受体位点。我们分析了hGH基因序列，以解释仅在此位置发生的高频率可变剪接现象。在本研究中，我们提出CTTGNNPyPyPy作为指导分支核苷酸选择的另一个共有序列。
