Jackson-Grusby L L, Swiergiel J, Linzer D I
Nucleic Acids Res. 1987 Aug 25;15(16):6677-90. doi: 10.1093/nar/15.16.6677.
We have characterized an mRNA that increases in abundance after serum stimulation of quiescent mouse fibroblasts. This mRNA, designated 18A2, encodes a predicted polypeptide of 101 amino acids with homology to known calcium binding proteins. A variety of mouse tissues express the 18A2 mRNA, with the highest levels detected in the non-pregnant uterus and in the placenta. The concentration of 18A2 mRNA in total placental RNA decreases from day 8 to day 10 of pregnancy, and is below detection throughout the latter half of gestation. In serum-stimulated fibroblasts, the increase in 18A2 mRNA is dependent on protein synthesis. The 18A2 mRNA is similar in size, serum-inducibility, and sequence to the 2A9 mRNA (1), but these mRNAs are derived from distinct genes. This suggests that the mouse genome harbors a family of serum-inducible genes encoding proteins predicted to bind calcium.
我们已经鉴定出一种在血清刺激静止的小鼠成纤维细胞后丰度增加的mRNA。这种mRNA被命名为18A2，编码一个预测的由101个氨基酸组成的多肽，与已知的钙结合蛋白具有同源性。多种小鼠组织表达18A2 mRNA，在未孕子宫和胎盘中检测到的水平最高。在怀孕第8天到第10天，胎盘总RNA中18A2 mRNA的浓度下降，并且在妊娠后半期一直低于检测水平。在血清刺激的成纤维细胞中，18A2 mRNA的增加依赖于蛋白质合成。18A2 mRNA在大小、血清诱导性和序列方面与2A9 mRNA（1）相似，但这些mRNA来自不同的基因。这表明小鼠基因组含有一个血清诱导基因家族，这些基因编码预测能结合钙的蛋白质。
