Linzer D I, Nathans D
EMBO J. 1985 Jun;4(6):1419-23. doi: 10.1002/j.1460-2075.1985.tb03796.x.
Mouse placenta has been found to contain an mRNA that encodes a previously unidentified member of the prolactin-growth hormone family. This 1.1-kb mRNA (designated PRP mRNA) was detected as a cDNA clone that hydridized to a cDNA clone of mouse proliferin, a recently described growth-associated placental protein related to prolactin. PRP mRNA levels are highest in the fetal part of the placenta and peak at day 12 of gestation, decreasing gradually until term. The 972-bp sequence of PRP mRNA, determined from two cDNA clones, encodes a protein of 244 amino acid residues that has a hydrophobic leader sequence. The protein encoded by PRP mRNA has significant homology to all of the members of the prolactin family, yet is different from each of them; it also differs from mouse placental lactogen. Nucleotide sequence homology is most extensive between PRP and proliferin mRNAs, particularly at their 5' ends, where they share 92 of the first 97 nucleotides.
已发现小鼠胎盘含有一种信使核糖核酸（mRNA），它编码催乳素 - 生长激素家族中一个此前未被鉴定的成员。这种1.1千碱基的mRNA（命名为PRP mRNA）作为一个互补DNA（cDNA）克隆被检测到，该克隆与小鼠增殖蛋白的一个cDNA克隆杂交，小鼠增殖蛋白是最近描述的一种与催乳素相关的生长相关胎盘蛋白。PRP mRNA水平在胎盘的胎儿部分最高，在妊娠第12天达到峰值，然后逐渐下降直至足月。从两个cDNA克隆确定的PRP mRNA的972碱基序列编码一个含有244个氨基酸残基且具有疏水前导序列的蛋白质。PRP mRNA编码的蛋白质与催乳素家族的所有成员都有显著的同源性，但又与它们中的每一个不同；它也不同于小鼠胎盘催乳素。PRP和增殖蛋白mRNA之间的核苷酸序列同源性最为广泛，特别是在它们的5'端，在前97个核苷酸中它们有92个相同。
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