College of Biosystems Engineering and Food Science, National-Local Joint Engineering Laboratory of Intelligent Food Technology and Equipment, Zhejiang Key Laboratory for Agro-Food Processing, Zhejiang University, Hangzhou 310058, Ningbo Innovation Center, Zhejiang University, Ningbo 315100, China; College of Food Science and Engineering, Production & Construction Group Key Laboratory of Special Agricultural Products Further Processing in Southern Xinjiang, Tarim University, Alar 843300, China.
College of Biosystems Engineering and Food Science, National-Local Joint Engineering Laboratory of Intelligent Food Technology and Equipment, Zhejiang Key Laboratory for Agro-Food Processing, Zhejiang University, Hangzhou 310058, Ningbo Innovation Center, Zhejiang University, Ningbo 315100, China.
Int J Biol Macromol. 2023 Jan 1;224:776-785. doi: 10.1016/j.ijbiomac.2022.10.165. Epub 2022 Oct 22.
Two neutral Glycyrrhiza polysaccharide (GP), named GP-LA and GP-HA, with molecular weights of 3.023 × 10 and 1.291 × 10, respectively, were extracted from Glycyrrhiza residues by hot acid extraction (HA) and low-temperature acid extraction (LA) and purified by column chromatography. Comprehensive analysis showed that the backbone of GP was composed of →4)-β-D-Glcp-(1→. For GP-LA, the side chain probably composed of β-L-Araf-(1→, →5)-β-L-Araf-(1→, →4)-β-D-Xylp-(1→, β-D-Glcp-(1→, →3,4)-α-D-Glcp-(1→, →4,6)-α- D-Glcp-(1→, and for GP-LA, the side chain probably composed of→6)-β-D-Galp-(1→, -α-D-Glcp-(1→, →3,4)-α-D-Glcp-(1→, →2,4)-α-D-Glcp-(1→, →4,6)- D-Glcp-(1→. The GP had a triple helix conformation and numerous irregular spherical particles with smooth surfaces. Atomic force microscopy (AFM) confirmed that GP-HA possessed single-branched structures and GP-LA possessed cross-linked network structures. X-ray diffraction (XRD) analysis revealed that GP-LA had both crystalline and amorphous structures, but GP-HA had no crystal structure. Furthermore, the results indicated that GP-LA and GP-HA exhibited certain inhibition activity on α-glucosidase. CD spectra and fluorescence intensity measurements confirmed that the secondary structure of α-glucosidase changed with the addition of GP.
两种中性甘草多糖（GP），分别命名为 GP-LA 和 GP-HA，分子量分别为 3.023×10 和 1.291×10，通过热酸提取（HA）和低温酸提取（LA）从甘草残渣中提取，并通过柱色谱法纯化。综合分析表明，GP 的骨架由→4）-β-D-Glcp-（1→组成。对于 GP-LA，侧链可能由β-L-Araf-（1→、→5）-β-L-Araf-（1→、→4）-β-D-Xylp-（1→、β-D-Glcp-（1→、→3,4）-α-D-Glcp-（1→、→4,6）-α-D-Glcp-（1→和 GP-LA，侧链可能由→6）-β-D-Galp-（1→、-α-D-Glcp-（1→、→3,4）-α-D-Glcp-（1→、→2,4）-α-D-Glcp-（1→、→4,6）-D-Glcp-（1→组成。GP 具有三螺旋构象和许多表面光滑的不规则球形颗粒。原子力显微镜（AFM）证实 GP-HA 具有单分支结构，GP-LA 具有交联网络结构。X 射线衍射（XRD）分析表明，GP-LA 具有结晶和无定形结构，但 GP-HA 没有晶体结构。此外，结果表明 GP-LA 和 GP-HA 对α-葡萄糖苷酶具有一定的抑制活性。CD 光谱和荧光强度测量证实，随着 GP 的加入，α-葡萄糖苷酶的二级结构发生了变化。
