莲雾果实多糖的结构表征、理化性质及α-葡萄糖苷酶抑制活性研究。
Structural characterization, physicochemical properties and α-glucosidase inhibitory activity of polysaccharide from the fruits of wax apple.
机构信息
Engineering Research Center of Bio-process of Ministry of Education, Hefei University of Technology, Hefei, Anhui 230009, PR China; School of Food and Biological Engineering, Hefei University of Technology, 193 Tunxi Road, Hefei, Anhui 230009, PR China.
Engineering Research Center of Bio-process of Ministry of Education, Hefei University of Technology, Hefei, Anhui 230009, PR China; School of Food and Biological Engineering, Hefei University of Technology, 193 Tunxi Road, Hefei, Anhui 230009, PR China.
出版信息
Carbohydr Polym. 2019 May 1;211:227-236. doi: 10.1016/j.carbpol.2019.02.006. Epub 2019 Feb 4.
In this study, a novel water-soluble polysaccharide fraction (WAFP) was isolated from wax apple fruits. The structural characterization, physicochemical properties and α-glucosidase inhibitory activity of WAFP were investigated. The results indicated that WAFP had average molecular weight of 49.9 kDa, it was mainly composed of rhamnose, arabinose, xylose, mannose, glucose, and galactose with a molar ratio of 1.88: 2.20: 6.37: 1.48: 5.47: 2.82. Besides, the backbone of WAFP might consist of →4)-α-D-Glcp-(1→, →3,4)-β-D-Xylp-(1→ and →3)-β-D-Galp-(1→, and the side chains might be composed of →3,5)-α-L-Araf-(1→, T-α-D-Glcp, →3)-β-D-Manp-(1→, →4)-α-D-GalpA-(1→ and →2)-α-L-Rhap-(1→. AFM images revealed that the height of WAFP molecules was about 12.8 nm. WAFP had no triple-helix structure, and it exhibited a shear-thinning behavior in the solution. SEM results showed that WAFP presented loose and incompact appearance and rough surface with many small irregular particles existed in the state of aggregation. Moreover, WAFP showed significant inhibitory effect on the activity of α-glucosidase.
本研究从莲雾果实中分离得到一种新型水溶性多糖(WAFP)。对 WAFP 的结构特征、理化性质和α-葡萄糖苷酶抑制活性进行了研究。结果表明，WAFP 的平均分子量为 49.9kDa，主要由鼠李糖、阿拉伯糖、木糖、甘露糖、葡萄糖和半乳糖组成，摩尔比为 1.88:2.20:6.37:1.48:5.47:2.82。此外，WAFP 的糖链骨架可能由→4)-α-D-Glcp-(1→、→3,4)-β-D-Xylp-(1→和→3)-β-D-Galp-(1→组成，侧链可能由→3,5)-α-L-Araf-(1→、T-α-D-Glcp、→3)-β-D-Manp-(1→、→4)-α-D-GalpA-(1→和→2)-α-L-Rhap-(1→组成。原子力显微镜(AFM)图像显示，WAFP 分子的高度约为 12.8nm。WAFP 没有三螺旋结构，在溶液中表现出剪切变稀行为。SEM 结果表明，WAFP 呈现出疏松、不紧密的外观，表面粗糙，存在许多小的不规则颗粒，处于聚集状态。此外，WAFP 对α-葡萄糖苷酶活性表现出显著的抑制作用。
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