State Key Laboratory of Food Nutrition and Safety, Tianjin University of Science and Technology, Tianjin 300457, PR China; Key Laboratory of Food Nutrition and Safety, Ministry of Education, Tianjin University of Science and Technology, Tianjin 300457, PR China; College of Food Science and Biotechnology, Tianjin University of Science and Technology, Tianjin 300457, PR China.
State Key Laboratory of Food Nutrition and Safety, Tianjin University of Science and Technology, Tianjin 300457, PR China; Key Laboratory of Food Nutrition and Safety, Ministry of Education, Tianjin University of Science and Technology, Tianjin 300457, PR China; College of Food Science and Biotechnology, Tianjin University of Science and Technology, Tianjin 300457, PR China.
Int J Biol Macromol. 2020 Aug 1;156:1512-1519. doi: 10.1016/j.ijbiomac.2019.11.199. Epub 2019 Nov 27.
The Pleurotus eryngii was chose as research subject to study the similarity and discrepancy of polysaccharides from fruiting body and mycelia, which were named as PEBP-II and PEMP-II, respectively. The purpose is to expand the production and application of Pleurotus eryngii polysaccharide. The molecular weights of PEBP-II and PEMP-II were 4.062 × 10 Da and 4.189 × 10 Da, respectively. The PEBP-II was composed of α-d-Glcp(1→, →6)-β-d-Galp(1→, →4)-α-d-Glcp(1→, →3,4)-α-d-Glcp(1→ and →3)-α-d-Manp(1→. The PEMP-II was composed of α-d-Glcp(1→, →6)-β-d-Galp(1→,→4)-α-d-Glcp(1→, →3,4)-α-d-Glcp(1→, →3)-α-d-Manp(1→, →3, 6)-α-d-Manp(1→ and →3)-α-d-Glcp(1→. The PEBP-II and PEMP-II both had dendritic and filamentous structure. The difference of PEBP-II and PEMP-II in structure was the ratio and kinds of backbone and branches. The PEBP-II and PEMP-II had equivalent inhibition on α-glucosidase and can significant inhibit the activity of α-glucosidase with inhibition types of competitive. All the results revealed that the polysaccharides from mycelia can be an effective substitute of polysaccharides from fruiting body. The manuscript expanded the production and application of Pleurotus eryngii polysaccharide. The analysis of chemical structure can provide theoretical basis for exploring the structure-activity relationship of polysaccharides.
杏鲍菇被选为研究对象，以研究其子实体和菌丝体多糖（分别命名为 PEBP-II 和 PEMP-II）的相似性和差异，目的是扩大杏鲍菇多糖的生产和应用。PEBP-II 和 PEMP-II 的分子量分别为 4.062×10^4 Da 和 4.189×10^4 Da。PEBP-II 由 α-d-Glcp(1→, →6)-β-d-Galp(1→, →4)-α-d-Glcp(1→, →3,4)-α-d-Glcp(1→ 和 →3)-α-d-Manp(1→ 组成。PEMP-II 由 α-d-Glcp(1→, →6)-β-d-Galp(1→,→4)-α-d-Glcp(1→, →3,4)-α-d-Glcp(1→, →3)-α-d-Manp(1→, →3, 6)-α-d-Manp(1→ 和 →3)-α-d-Glcp(1→ 组成。PEBP-II 和 PEMP-II 均具有树枝状和丝状结构。PEBP-II 和 PEMP-II 在结构上的差异在于主链和支链的比例和种类。PEBP-II 和 PEMP-II 对α-葡萄糖苷酶均具有同等的抑制作用，并且均可以显著抑制α-葡萄糖苷酶的活性，抑制类型为竞争性。所有结果表明，菌丝体多糖可以作为子实体多糖的有效替代品。本文扩大了杏鲍菇多糖的生产和应用。化学结构分析可为探索多糖的结构-活性关系提供理论依据。
