Department of Biochemistry, Punjab Agricultural University, Ludhiana, Punjab, India.
Department of Vegetable Science, Punjab Agricultural University, Ludhiana, Punjab, India.
Physiol Plant. 2022 Sep;174(5):e13756. doi: 10.1111/ppl.13756.
Solanum melongena is a widely consumed vegetable crop comprising health-benefiting phenolic compounds. It has a complex network of biosynthetic enzymes involved in synthesizing nutraceuticals, including anthocyanins. The present study was conducted to investigate the activities of key enzymes involved in biosynthesis and accumulation of anthocyanins in developing genotypes, such as phenylalanine ammonia lyase (PAL), tyrosine ammonia lyase (TAL), and anthocyanidin synthase (ANS). As inadequate information is available in this context, fruit and leaf tissues were analyzed for enzyme activities and anthocyanin accumulation. The study included characterization of extracted anthocyanin followed by expression studies for gateway enzyme (ANS) involved in anthocyanin biosynthesis. Delphinidin was a major anthocyanidin present in fruit tissues (1.46-110.49 mg/100 g) of S. melongena. Anthocyanin accumulation is backed up by the correlation between biochemical analysis and expression studies. The study has shown variation for PAL, TAL and ANS enzymes in different tissues at developmental stages. Enzyme activities had a strong positive correlation with anthocyanin biosynthesis.
茄子是一种广泛食用的蔬菜作物，含有有益健康的酚类化合物。它有一个复杂的生物合成酶网络，参与合成营养保健品，包括花青素。本研究旨在研究不同发育基因型中参与花青素生物合成和积累的关键酶的活性，如苯丙氨酸解氨酶（PAL）、酪氨酸解氨酶（TAL）和花青素合酶（ANS）。由于这方面的信息不足，对果实和叶片组织进行了酶活性和花青素积累分析。该研究包括对提取的花青素进行表征，然后对参与花青素生物合成的门控酶（ANS）进行表达研究。果实组织中主要的花青素是矢车菊素（1.46-110.49mg/100g）。生化分析和表达研究表明花青素的积累。研究表明，PAL、TAL 和 ANS 酶在不同组织和发育阶段存在差异。酶活性与花青素生物合成呈强正相关。
