Zebrafish Genetics Laboratory, Department of Genetic Engineering, SRM Institute of Science and Technology, Kattankulathur, India.
Zebrafish. 2022 Oct;19(5):177-180. doi: 10.1089/zeb.2022.0023.
Zebrafish larva is a simple model system to study blood vessel development because of its excellent optical properties. Current methods to study blood vessels in zebrafish involve utilizing either blood vessel-specific transgenic lines or specialized techniques such as microangiography and video capillaroscopy. In this study, we have developed a simple protocol using ImageJ to observe the blood vessels of live zebrafish larva at different developmental stages, namely 1- to 4-day postfertilization (dpf). To validate this protocol, we treated 1 dpf zebrafish embryos with 100 μM quercetin, and analyzed the impaired development of subintestinal vein in the quercetin-treated 3 dpf larvae.
斑马鱼幼虫因其出色的光学特性，是研究血管发育的简单模式系统。目前研究斑马鱼血管的方法包括利用血管特异性转基因系或微血管造影和视频毛细血管镜等专门技术。在这项研究中，我们使用 ImageJ 开发了一种简单的方案，用于观察不同发育阶段（即受精后 1 至 4 天，1-4 dpf）的活体斑马鱼幼虫的血管。为了验证该方案，我们用 100 μM 槲皮素处理 1 dpf 的斑马鱼胚胎，并分析了在 3 dpf 幼虫中，槲皮素处理导致的亚肠静脉发育受损情况。
