活体和固定斑马鱼胚胎/幼鱼的血管生成分析。

Angiogenesis Assay for Live and Fixed Zebrafish Embryos/Larvae.

机构信息

Zebrafish Genetics Laboratory, Department of Genetic Engineering, SRM Institute of Science and Technology, Kattankulathur, Chengalpattu, Tamil Nadu, India.

Molecular Toxicology Laboratory, Department of Biotechnology, Bharathiar University, Coimbatore, Tamil Nadu, India.

出版信息

Methods Mol Biol. 2024;2753:377-384. doi: 10.1007/978-1-0716-3625-1_21.

DOI:10.1007/978-1-0716-3625-1_21

PMID:38285352

Abstract

Angiogenesis is the process of new blood vessel formation from preexisting vasculature. It is an integral component in normal embryonic development and tissue repair. Dysregulation of angiogenesis might lead to tissue ischemia (resulting from reduced blood vessel formation) or major diseases such as cancer (abnormal vascular growth). This makes angiogenesis an excellent area of research for cancer therapeutics, and various animal models including zebrafish are used to study blood vessel development. As most of the techniques used to study angiogenesis are complex and cumbersome, in this chapter, we provide two simple assays to study angiogenesis with live and fixed zebrafish embryos/larvae.

摘要

血管生成是指从预先存在的脉管系统中形成新的血管的过程。它是正常胚胎发育和组织修复的一个组成部分。血管生成的失调可能导致组织缺血（由于血管生成减少所致）或癌症等重大疾病（异常血管生长）。这使得血管生成成为癌症治疗学的一个极好的研究领域，并且使用各种动物模型包括斑马鱼来研究血管发育。由于用于研究血管生成的大多数技术复杂而繁琐，因此在本章中，我们提供了两种使用活体和固定斑马鱼胚胎/幼虫研究血管生成的简单测定法。