Department of Wellness-Bio Industry, Gangneung-Wonju National University, Gangneung, Gangwon, 25457, South Korea.
Department of Marine Food Science and Technology, Gangneung-Wonju National University, Gangneung, Gangwon, 25457, South Korea.
Fish Shellfish Immunol. 2022 Dec;131:1109-1117. doi: 10.1016/j.fsi.2022.10.039. Epub 2022 Oct 23.
In the present study, total lipids were extracted from Ammodytes personatus eggs and separated into neutral lipids, glycolipids, and phospholipids. The anti-inflammatory activity of the neutral lipids, glycolipids, and phospholipids was investigated in macrophages, as well as the fatty acid profiles of the lipids. Palmitic acid, oleic acid, docosahexaenoic acid (DHA), and eicosapentaenoic acid (EPA) were the primary fatty acids in the three fractionated lipids. Among the lipids, the phospholipids contained the highest concentration of polyunsaturated fatty acids (PUFAs), particularly DHA and EPA (31.89 and 16.93% of the total fatty acids, respectively). The anti-inflammatory effects of the three lipids isolated from A. personatus eggs were analyzed in lipopolysaccharide (LPS)-stimulated RAW264.7 cells. The three lipids significantly reduced nitric oxide (NO) production and the mRNA expression of immune-associated genes in a dose-dependent manner. All lipids down-regulated the protein expression of phosphorylated NF-κB-p65 and MAPK (p38, JNK, and ERK1/2) signaling pathways, suggesting that they could inhibit cell signaling pathways by activating NF-κB and MAPK. The expression of CD40 and CD86 in LPS-stimulated RAW264.7 cells was also significantly decreased by A. personatus lipids. Consequently, the neutral lipids, glycolipids, and phospholipids from A. personatus eggs could serve as anti-inflammatory agents.
在本研究中，从毛鳞鱼卵中提取总脂质，并将其分离为中性脂质、糖脂和磷脂。研究了中性脂质、糖脂和磷脂在巨噬细胞中的抗炎活性，以及脂质的脂肪酸谱。棕榈酸、油酸、二十二碳六烯酸（DHA）和二十碳五烯酸（EPA）是这三种分离脂质的主要脂肪酸。在这三种脂质中，磷脂含有最高浓度的多不饱和脂肪酸（PUFAs），特别是 DHA 和 EPA（分别占总脂肪酸的 31.89%和 16.93%）。分析了毛鳞鱼卵中分离得到的三种脂质在脂多糖（LPS）刺激的 RAW264.7 细胞中的抗炎作用。这三种脂质以剂量依赖的方式显著降低了一氧化氮（NO）的产生和免疫相关基因的 mRNA 表达。所有脂质均下调磷酸化 NF-κB-p65 和 MAPK（p38、JNK 和 ERK1/2）信号通路的蛋白表达，表明它们可以通过激活 NF-κB 和 MAPK 来抑制细胞信号通路。LPS 刺激的 RAW264.7 细胞中 CD40 和 CD86 的表达也显著降低。因此，毛鳞鱼卵的中性脂质、糖脂和磷脂可作为抗炎剂。
