Department of Wellness-Bio Industry, Gangneung-Wonju National University, Gangneung, Gangwon 25457, Korea.
Department of Marine Food Science and Technology, Gangneung-Wonju National University, Gangneung, Gangwon 25457, Korea.
Molecules. 2021 Oct 4;26(19):6027. doi: 10.3390/molecules26196027.
, known as the Pacific sand lance, thrives in cold areas of the North Pacific. In this study, the total lipid was extracted from eggs and the fatty acid composition was determined using gas chromatography (GC)-flame ionization detection (FID). The results showed that the extracted lipid contained high content of polyunsaturated fatty acids (PUFAs). The immunomodulatory activities of the lipid were investigated using rodent macrophages. First, immune enhancement was analyzed, and the lipid significantly and dose-dependently increased the NO production in RAW264.7 cells, and this lipid also regulated the transcription of immune-associated genes in RAW264.7 cells by activating the NF-κB and MAPK pathways. Additionally, flow cytometry revealed that this lipid stimulated phagocytosis. Conversely, the anti-inflammatory activity of the lipid was also analyzed and the results showed significantly decreased NO production and gene expression in a dose-dependent manner in LPS-stimulated RAW264.7 cells. In addition, the lipid suppressed the LPS-induced phosphorylation of proteins related to the NF-κB and MAPK pathways in LPS-stimulated RAW264.7 cells. Further, flow cytometry demonstrated the lipid-regulated anti-inflammatory activity via inhibition of CD86 expression. The results indicate that egg lipid is a potential source of immunomodulation.
太平洋磷沙蚕，俗称太平洋玉筋鱼，盛产于北太平洋寒冷地区。本研究采用气相色谱（GC）-火焰离子化检测（FID）法测定其卵脂的脂肪酸组成。结果表明，提取的脂类含有丰富的多不饱和脂肪酸（PUFAs）。采用鼠源巨噬细胞研究了该脂类的免疫调节活性。首先，分析了其免疫增强作用，结果表明该脂类能显著且剂量依赖性地增加 RAW264.7 细胞中 NO 的产生，还通过激活 NF-κB 和 MAPK 通路调节 RAW264.7 细胞中与免疫相关的基因转录。此外，流式细胞术显示该脂类能刺激吞噬作用。相反，还分析了该脂类的抗炎活性，结果表明该脂类能显著抑制 LPS 刺激的 RAW264.7 细胞中 NO 的产生和基因表达，并呈剂量依赖性。此外，该脂类还能抑制 LPS 刺激的 RAW264.7 细胞中与 NF-κB 和 MAPK 通路相关蛋白的磷酸化。进一步的流式细胞术研究表明，该脂类通过抑制 CD86 的表达来调节抗炎活性。这些结果表明，太平洋磷沙蚕卵脂是一种具有免疫调节潜力的潜在来源。
