Department of Marine Biotechnology, Gangneung-Wonju National University, Gangneung, Gangwon 25457, Korea.
East Coast Life Sciences Institute, Gangneung-Wonju National University, Gangneung, Gangwon 25457, Korea.
Mar Drugs. 2020 Sep 21;18(9):480. doi: 10.3390/md18090480.
Total lipids were extracted from sandfish (), and then they were separated into the following three lipid fractions: neutral lipids, glycolipids, and phospholipids. In this study, we analyzed the lipid fractions of eggs and we determined their anti-inflammatory activity in RAW264.7 macrophage cells. In these three lipid-fractions, the main fatty acids were as follows: palmitic acid (16:0), oleic acid (18:1n-9), docosahexaenoic acid (DHA, 22:6n-3), and eicosapentaenoic acid (EPA, 20:5n-3). Among the lipid fractions, phospholipids showed the highest concentration of DHA and EPA (21.70 ± 1.92 and 18.96 ± 1.27, respectively). The three lipid fractions of significantly suppressed the production of NO in macrophages. Moreover, they also significantly inhibited the expression of , , , , and , in a dose-dependent manner. Furthermore, the lipid fractions of suppressed the nuclear translocation of NF-κB p65 subunits in a dose-dependent manner. In addition, they attenuated the activation of MAPKs (p38, ERK1/2, and JNK) phosphorylation in LPS-stimulated RAW264.7 cells. These results indicate that all the lipid fractions of exert anti-inflammatory activity by suppressing the activation of NF-κB and MAPK pathways. Therefore, the lipid fractions of might be potentially used as anti-inflammatory agents.
从沙鱼（）中提取总脂质，然后将其分为以下三种脂质馏分：中性脂质、糖脂和磷脂。在这项研究中，我们分析了卵的脂质馏分，并在 RAW264.7 巨噬细胞中测定了它们的抗炎活性。在这三种脂质馏分中，主要脂肪酸如下：棕榈酸（16:0）、油酸（18:1n-9）、二十二碳六烯酸（DHA，22:6n-3）和二十碳五烯酸（EPA，20:5n-3）。在脂质馏分中，磷脂显示出最高浓度的 DHA 和 EPA（分别为 21.70 ± 1.92 和 18.96 ± 1.27）。卵的三种脂质馏分显著抑制巨噬细胞中 NO 的产生。此外，它们还以剂量依赖性方式显著抑制 、 、 、 和 的表达。此外，卵的脂质馏分还以剂量依赖性方式抑制 NF-κB p65 亚基的核易位。此外，它们还减弱了 LPS 刺激的 RAW264.7 细胞中 MAPKs（p38、ERK1/2 和 JNK）磷酸化的激活。这些结果表明，卵的所有脂质馏分通过抑制 NF-κB 和 MAPK 途径的激活发挥抗炎活性。因此，卵的脂质馏分可能有潜力用作抗炎剂。
