Department of Biotechnology, College of Sciences, Taif University, Taif 21944, Saudi Arabia.
Department of Biosciences, Faculty of Life Sciences, Shaheed Zulfikar Ali Bhutto Institute of Science and Technology, Karachi 75600, Pakistan.
Genes (Basel). 2023 Mar 24;14(4):783. doi: 10.3390/genes14040783.
Pyoderma is a common skin infection predominantly caused by In addition to methicillin resistance, this pathogen is resistant to many other antibiotics, which ultimately limits the available treatment options. Therefore, the present study aimed to compare the antibiotic-resistance pattern, to detect the gene and the genes encoding microbial surface component recognizing adhesive matrix molecules (MSCRAMMs) in isolates. A total of 116 strains were isolated from patients suffering with pyoderma. Disk diffusion assay was opted to perform antimicrobial susceptibility testing of the isolates. Out of the isolates tested, 23-42.2% strains appeared susceptible to benzylpenicillin, cefoxitin, ciprofloxacin and erythromycin. While linezolid was found to be the most effective anti-staphylococcal drug, followed by rifampin, chloramphenicol, clindamycin, gentamicin and ceftaroline. Out of 116 isolates, 73 (62.93%) were methicillin-resistant (MRSA). Statistically significant ( ≤ 0.05) differences in antibiotic resistance patterns between MRSA and methicillin-susceptible (MSSA) were found. A significant association of resistance to ceftaroline, rifampin, tetracycline, ciprofloxacin, clindamycin, trimethoprim-sulfamethoxazole and chloramphenicol was found in MRSA. However, no significant difference was observed between MRSA and MSSA for resistance against gentamicin, erythromycin or linezolid. All cefoxitin-resistant , nonetheless, were positive for the gene. was found in all the MRSA isolates. Among other virulence markers, and were found in all the isolates, while (98.3%), and (99.1%) were present predominately in MRSA. Thus, this study offers an understanding of antibiotic resistance MSCRAMMs, , and gene patterns in locally isolated strains of .
脓疱病是一种常见的皮肤感染，主要由 引起。除了耐甲氧西林外，这种病原体还对许多其他抗生素具有耐药性，这最终限制了可用的治疗选择。因此，本研究旨在比较抗生素耐药模式，检测 基因和编码微生物表面成分识别黏附基质分子（MSCRAMMs）的基因在 分离株中的分布。从患有脓疱病的患者中分离出 116 株菌株。采用纸片扩散法对抗生素敏感性进行了测试。在所测试的分离株中，23-42.2%的菌株对苯唑西林、头孢西丁、环丙沙星和红霉素表现出敏感性。而利奈唑胺被发现是最有效的抗葡萄球菌药物，其次是利福平、氯霉素、克林霉素、庆大霉素和头孢洛林。在 116 株分离株中，73 株（62.93%）为耐甲氧西林金黄色葡萄球菌（MRSA）。MRSA 和甲氧西林敏感金黄色葡萄球菌（MSSA）之间的抗生素耐药模式存在统计学显著差异（≤0.05）。在 MRSA 中发现对头孢洛林、利福平、四环素、环丙沙星、克林霉素、复方磺胺甲噁唑和氯霉素的耐药性存在显著相关性。然而，MRSA 和 MSSA 之间对庆大霉素、红霉素或利奈唑胺的耐药性没有差异。所有头孢西丁耐药的 ，但都呈阳性。在所有的 MRSA 分离株中都发现了 基因。在其他毒力标志物中，所有分离株均发现了 和 ，而 （98.3%）和 （99.1%）主要存在于 MRSA 中。因此，本研究有助于了解本地分离株中 的抗生素耐药性、MSCRAMMs、 基因和 基因模式。
