State Key Laboratory of Bioelectronics, School of Biological Science and Medical Engineering, Southeast University, Nanjing210096, China.
Anal Chem. 2022 Nov 15;94(45):15809-15817. doi: 10.1021/acs.analchem.2c03582. Epub 2022 Oct 26.
A novel efficacious strategy for real-time monitoring of the release of hydrophobic cargo curcumin (molecule model nutraceuticals) from a lipid-curcumin-loaded silica colloidal crystal (L(Cur)-SCC) film controlled by lipase was developed. Curcumin was dispersed in a proportion of a digestible lipid complex (glycerol trioleate and glycerol tristearate) to prepare a lipid-curcumin complex and then loaded into the SCC film by a capillary to prepare an L(Cur)-SCC film. Lipase-triggered degradation of the digestible lipid complex resulted in curcumin release being tracked in real-time by ordered porous layer interferometry (OPLI). The optical thickness changes (ΔOT) of the L(Cur)-SCC film depend on the mass changes of the lipid-curcumin complex due to the migration of interference fringes caused by the lipase degradation of the digestible lipid complex. Curcumin release from the L(Cur)-SCC film was characterized and analyzed in combination with an ultraviolet-visible spectrophotometer, a nanoparticle size analyzer, and an attenuated total reflection infrared spectrometer. The introduction of a soluble dietary fiber (pectin) into the L(Cur)-SCC film delayed the release rate of curcumin. Furthermore, the real-time sustained release of curcumin from the L(Cur)-SCC film in the simulated digestive fluids was tracked. This study provides an early exploration of the real-time controlled release of lipid-soluble nutraceuticals in the gastrointestinal tract.
一种新型有效策略被开发用于实时监测疏水性货物姜黄素（分子模型营养保健品）从由脂肪酶控制的载有姜黄素的硅质胶体晶体（L(Cur)-SCC）膜中的释放。姜黄素分散在可消化脂质复合物（三油酸甘油酯和三硬脂酸甘油酯）中以制备脂质-姜黄素复合物，然后通过毛细管将其载入 SCC 膜中以制备 L(Cur)-SCC 膜。可消化脂质复合物的脂肪酶触发降解导致姜黄素释放被有序多孔层干涉测量法（OPLI）实时跟踪。L(Cur)-SCC 膜的光学厚度变化（ΔOT）取决于脂质-姜黄素复合物的质量变化，这是由于可消化脂质复合物的脂肪酶降解引起的干涉条纹的迁移所致。结合紫外可见分光光度计、纳米颗粒粒度分析仪和衰减全反射红外光谱仪对 L(Cur)-SCC 膜中的姜黄素释放进行了表征和分析。在 L(Cur)-SCC 膜中引入可溶膳食纤维（果胶）会延迟姜黄素的释放速率。此外，还跟踪了 L(Cur)-SCC 膜中姜黄素在模拟消化液中的实时持续释放。本研究为探索脂溶性营养保健品在胃肠道中的实时控制释放提供了早期探索。
