Zhou Lele, Bian Haixin, Zhang Na, Qian Weiping
School of Pharmacy, Binzhou Medical University, Yantai 264003, China.
School of Pharmacy, Binzhou Medical University, Yantai 264003, China.
Food Chem. 2024 Jun 30;444:138581. doi: 10.1016/j.foodchem.2024.138581. Epub 2024 Jan 30.
The model proteins bovine serum albumin (BSA) and lipid layer were used to study the effect of proteins on lipolysis. A lipid layer with an interference effect was constructed by loading the triolein into the silica colloidal crystal (SCC) film. The ordered porous layer interferometry (OPLI) system was used to track the changes in lipid layer mass caused by lipase hydrolysis to achieve real-time lipolysis detection. The real-time tracking of the adsorption of BSA on the lipid layer by converting the migration of interference fringes caused by the change of the lipid layer into the optical thickness change (ΔOT). The effect of BSA on the early and late stages of lipolysis was studied, and lipases containing 5 mg/mL BSA degraded the lipid layer 3.4 times faster than lipases containing 0.1 mg/mL BSA in the later stages. This study deepens the understanding of protein-lipid interactions in complex digestive environments.
使用模型蛋白牛血清白蛋白（BSA）和脂质层来研究蛋白质对脂肪分解的影响。通过将三油酸甘油酯加载到二氧化硅胶体晶体（SCC）膜中构建具有干涉效应的脂质层。利用有序多孔层干涉测量法（OPLI）系统跟踪脂肪酶水解引起的脂质层质量变化，以实现实时脂肪分解检测。通过将脂质层变化引起的干涉条纹迁移转换为光学厚度变化（ΔOT），实时跟踪BSA在脂质层上的吸附。研究了BSA对脂肪分解早期和晚期的影响，在后期，含有5mg/mL BSA的脂肪酶降解脂质层的速度比含有0.1mg/mL BSA的脂肪酶快3.4倍。该研究加深了对复杂消化环境中蛋白质-脂质相互作用的理解。
